GRO-alpha in normal and pathological thyroid tissues and its regulation in thyroid-derived cells

Autor: C. Simchen, S. Kiessling, M Steinert, Gabriela Aust, C. Boltze
Rok vydání: 2001
Předmět:
Adenoma
Adult
Male
endocrine system
medicine.medical_specialty
Chemokine
endocrine system diseases
Endocrinology
Diabetes and Metabolism
Graves' disease
medicine.medical_treatment
Chemokine CXCL1
Population
Cell Culture Techniques
Thyroid Gland
Biology
Receptors
Interleukin-8B
Thyroid carcinoma
Endocrinology
Thyroid peroxidase
Internal medicine
medicine
Tumor Cells
Cultured
Humans
RNA
Messenger
Thyroid Neoplasms
education
Growth Substances
education.field_of_study
Chemotactic Factors
Reverse Transcriptase Polymerase Chain Reaction
Macrophages
Thyroid
Middle Aged
medicine.disease
Thyroid Diseases
Graves Disease
Neoplasm Proteins
medicine.anatomical_structure
Cytokine
Gene Expression Regulation
biology.protein
Intercellular Signaling Peptides and Proteins
lipids (amino acids
peptides
and proteins)
Female
Chemokines
CXC
Endocrine gland
Zdroj: The Journal of endocrinology. 170(3)
ISSN: 0022-0795
Popis: Thyroid glands affected by Graves' disease (GD) show striking leukocytic infiltration, mainly by T-cells. The mechanisms by which the various leukocytes are maintained in the thyroid are unknown. Growth-regulated oncogene-alpha (GRO-alpha) in interaction with its receptor CXCR2 is a chemoattractant for both T-cells and neutrophils and may be one of the chemokines involved in the cell maintenance. GRO-alpha and CD18 mRNA as a marker of leukocytic infiltration were quantified in thyroid tissue using competitive RT-PCR. We found very high GRO-alpha mRNA levels in all thyroid tissues. In GD patients (n=16), the GRO-alpha mRNA did not correlate with the CD18 mRNA level or thyroid peroxidase and TSH-receptor antibodies in patients' sera. In thyroid autonomy (n=10), the GRO-alpha mRNA levels were significantly lower in autonomous single adenomas compared with the corresponding normal tissue. In order to define the cellular source of GRO-alpha mRNA and protein, we examined various thyroid-derived cells. Thyrocytes, thyroid-derived leukocytes and fibroblasts showed basal GRO-alpha mRNA and protein expression, which was remarkably upregulated by different stimuli in vitro. The expression of GRO-alpha by thyroid carcinoma cell lines confirms that thyrocytes may actually produce GRO-alpha. As shown by flow cytometry and immunohistology, CD68+ monocytes/macrophages are the only cell population strongly expressing CXCR2 in the thyroid.
Databáze: OpenAIRE
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