Surface behaviour of bile salts and tetrahydrolipstatin at air/water and oil/water interfaces
| Autor: | Stéphane Ransac, Paul Hadvary, Hans Lengsfeld, Robert Verger, Ali Tiss, Alain Cagna |
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| Rok vydání: | 2001 |
| Předmět: |
Orlistat
Chromatography Surface Properties Chemistry Air Organic Chemistry Gastrointestinal Lipase Inhibitor Water Lipase Cell Biology Surface pressure Biochemistry Micelle Oil drop experiment Bile Acids and Salts Lactones Adsorption Chemical engineering Critical micelle concentration Monolayer Enzyme Inhibitors Oils Molecular Biology Micelles Wilhelmy plate |
| Zdroj: | Chemistry and Physics of Lipids. 111:73-85 |
| ISSN: | 0009-3084 |
| DOI: | 10.1016/s0009-3084(01)00149-9 |
| Popis: | The surface behaviour of two bile salts, sodium deoxycholate (NaDC) and sodium taurodeoxycholate (NaTDC), as well as that of tetrahydrolipstatin (THL), a potent gastrointestinal lipase inhibitor, was studied at air/water and oil/water interfaces, using interfacial tensiometry methods. The surface behaviour of NaDC and NaTDC was comparable at both oil/water and air/water interfaces. A fairly compact interfacial monolayer of bile salts is formed well below the critical micellar concentration (CMC) and can help to explain the well-known effects of bile salts on the kinetic behaviour of pancreatic lipases. Using the Wilhelmy plate technique, the surface pressure-molecular area curves recorded with THL at the air/water interface showed a collapse point at a surface pressure of 24.5 mN.m(-1), corresponding to a molecular area of 70 A(2). Surprisingly, using the oil drop method, a limiting molecular area of 160 A(2) was found to exist at the oil/water interface. On the basis of the above data, space-filling models were proposed for bile salts and THL at air/water and oil/water interfaces. |
| Databáze: | OpenAIRE |
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