Single-virus genomics and beyond
| Autor: | Francisco Martinez-Hernandez, Joaquín Martínez Martínez, Manuel Martinez-Garcia |
|---|---|
| Přispěvatelé: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología, Ecología Microbiana Molecular |
| Rok vydání: | 2020 |
| Předmět: |
Aquatic Organisms
Optical Tweezers Ecology (disciplines) viruses Cell Culture Techniques Genomics Genome Viral Computational biology Biology Microbiología Microbiology Genome Virus Carbon Cycle 03 medical and health sciences Animals Humans 0303 health sciences General Immunology and Microbiology 030306 microbiology Virus ecology Virion Computational Biology Genetic Variation Single-virus genomics Infectious Diseases Metagenomics Viruses Metagenome |
| Zdroj: | RUA. Repositorio Institucional de la Universidad de Alicante Universidad de Alicante (UA) |
| Popis: | Viruses are extremely diverse and modulate important biological and ecological processes globally. However, much of viral diversity remains uncultured and yet to be discovered. Several powerful culture-independent tools, in particular metagenomics, have substantially advanced virus discovery. Among those tools is single-virus genomics, which yields sequenced reference genomes from individual sorted virus particles without the need for cultivation. This new method complements virus culturing and metagenomic approaches and its advantages include targeted investigation of specific virus groups and investigation of genomic microdiversity within viral populations. In this Review, we provide a brief history of single-virus genomics, outline how this emergent method has facilitated advances in virus ecology and discuss its current limitations and future potential. Finally, we address how this method may synergistically intersect with other single-virus and single-cell approaches. This work was supported by the Gordon and Betty Moore Foundation (grant 5334), the US National Science Foundation (NSF- OPP 1644155, NSF- OCE 1933289), the Spanish Ministry of Economy and Competitiveness (CGL2013-40564- R, RTI2018-094248- B- I00 and SAF2013-49267- EXP) and Generalitat Valenciana (ACOM/2015/133 and ACIF/2015/332). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|