Effects of FSH treatment and withdrawal during proestrus on uterine proliferation and steroid hormone receptor expression in beef heifers
| Autor: | Suthipong Uriyapongson, Thanya Bunma, Chainarong Navanukraw, Nattawut Kogram, Chutikun Kanjanaruch, Vilaivan Khanthusaeng |
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| Rok vydání: | 2021 |
| Předmět: |
endocrine system
Steroid hormone receptor medicine.drug_class medicine.medical_treatment Estrogen receptor Gene Expression Estrous Cycle Andrology Follicle-stimulating hormone medicine Animals Saline Cell Proliferation Estrous cycle Cell growth business.industry Uterus Myometrium General Medicine Receptors Estrogen Cattle Female Gonadotropin Follicle Stimulating Hormone General Agricultural and Biological Sciences business hormones hormone substitutes and hormone antagonists |
| Zdroj: | Animal science journal = Nihon chikusan GakkaihoREFERENCES. 92(1) |
| ISSN: | 1740-0929 |
| Popis: | To determine the effects of Follicle Stimulating Hormone (FSH) treatment and subsequent withdrawal on uterine proliferation and estrogen receptor (ESR), Brahman crossbred heifers (n = 12) were twice daily injected with FSH (4, 3, and 2 mg/injection) on Days 17-19 of the estrous cycle (FSH 3 days) and (4 and 3 mg/injection) on Days 17-18 (FSH 2 days) and withdrawal with saline on Day 19 and (4 mg/injection) on Day 17 (FSH 1 day) and withdrawal with saline on Days 18-19. Uterine tissue was subjectively collected on Day 20 and microscopically classified to four regions: endometrial stroma (ES), surface endometrial gland (EG), deep endometrial gland (DG), and myometrium (Myo). The cell proliferation marker, Ki-67, was quantified as labeling index (LI) in uterine regions, and tissues were immunostained to detect ESR2 followed by image analysis. The LI of ES, EG, and DG was greater (P = 0.0018, P = 0.0005, and P = 0.0103; respectively) in heifers received FSH for 3 days. The expression of ESR2 protein on ES and EG was greatest (P < 0.0001 and P = 0.0036, respectively) in FSH 3 days-treated group. Thus, FSH administration during proestrus stimulates uterine cell proliferation, and ESR2 expressions are affected by FSH during proestrus and differentially distributed in the uterine regions. |
| Databáze: | OpenAIRE |
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