Gene expression profile of mouse bone marrow stromal cells determined by cDNA microarray analysis
| Autor: | Georg Wieczorek, Christine Steinhoff, Hans-Hilger Ropers, Ulrike A. Nuber, Martin Vingron, Ralph Schulz, Marina Scheller |
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| Rok vydání: | 2003 |
| Předmět: |
Cell type
Histology Stromal cell medicine.medical_treatment Bone Marrow Cells Cell Separation Biology Pathology and Forensic Medicine Extracellular matrix Mice medicine Animals Cloning Molecular Receptors Cytokine Cells Cultured DNA Primers Oligonucleotide Array Sequence Analysis Extracellular Matrix Proteins Base Sequence Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Mesenchymal stem cell Cell Biology Molecular biology Cytoskeletal Proteins Cytokine medicine.anatomical_structure Cytokines RNA Pericyte Bone marrow Stromal Cells Cell Adhesion Molecules Adult stem cell |
| Zdroj: | Cell and Tissue Research. 311:227-237 |
| ISSN: | 1432-0878 0302-766X |
| Popis: | Bone marrow stromal cells (BMSC) have gained increased attention because of their multipotency and adult stem cell character. They have been shown to differentiate into other cell types of the mesenchymal lineage and also into non-mesenchymal cells. The exact identity of the original cells, which are isolated from bone marrow by their selective adherence to plastic, remains unknown to date. We have established and characterized mouse BMSC cultures and analyzed three independent samples by cDNA microarrays. The expression profile was compared with two previous expression studies of human BMSC and revealed a high degree of concordance between different techniques and species. To gain clues about the positional context and biology of the isolated cells within the bone marrow stroma, we searched our data for genes that encode proteins of the extracellular matrix, cell adhesion proteins, cytoskeletal proteins and cytokines/cytokine receptors. This analysis revealed a close association of BMSC with vascular cells and indicated that BMSC resemble pericytes. |
| Databáze: | OpenAIRE |
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