Airway Eosinophilic Inflammation Is Attenuated in Conserved Noncoding Sequence-1-Deficient Mice
| Autor: | Yuichiro Kajiyama, Takayuki Ohtomo, Shoichiro Miyatake, Akio Mori, Noriaki Kobayashi, Makiko Umezu-Goto, Osamu Kaminuma |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_treatment
Immunology Inflammation Biology Conserved sequence Mice Th2 Cells Eosinophilia medicine Animals Immunology and Allergy Regulatory Elements Transcriptional Interleukin 5 Conserved Sequence Interleukin 4 Mice Knockout Interleukin-13 General Medicine T helper cell Immunoglobulin E Eosinophil Eosinophils Cytokine medicine.anatomical_structure Immunoglobulin G Interleukin 13 DNA Intergenic Interleukin-4 medicine.symptom Bronchoalveolar Lavage Fluid |
| Zdroj: | International Archives of Allergy and Immunology. 146:2-6 |
| ISSN: | 1423-0097 1018-2438 |
| DOI: | 10.1159/000126052 |
| Popis: | Background: Conserved noncoding sequence-1 (CNS-1) is an important regulatory element for T helper 2 cytokine expression. IL-4, IL-5 and IL-13 expression as well as serum IgE level were attenuated in CNS-1–/– mice. Method: CNS-1–/– and CNS-1+/+ mice were sensitized with ovalbumin (OVA) followed by antigen challenge. The number of eosinophils and T helper 2 cytokine concentration in the bronchoalveolar lavage fluid, OVA-specific IgE antibody (Ab) in the serum and bronchial responsiveness to methacholine were examined. Results: Bronchoalveolar lavage fluid eosinophilia was significantly attenuated in CNS-1–/– mice compared to CNS-1+/+ mice, which were sensitized with OVA/aluminum once. OVA-specific IgE Ab was also attenuated. When mice were sensitized with OVA/aluminum twice, induction of eosinophilia and OVA-specific IgE Ab was not significantly different between CNS-1–/– and CNS-1+/+ mice. Conclusion: CNS-1 locus regulates eosinophilic inflammation in vivo. |
| Databáze: | OpenAIRE |
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