Dye-permeable, voltage-gated channel on mouse fungiform taste bud cells
| Autor: | Keita Takeuchi, Yoshitaka Ohtubo, Yasuhisa Seto, Kiyonori Yoshii |
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| Rok vydání: | 2011 |
| Předmět: |
Time Factors
Sensory Receptor Cells Synaptosomal-Associated Protein 25 Voltage clamp Biophysics Phospholipase C beta Carbenoxolone 4 4'-Diisothiocyanostilbene-2 2'-Disulfonic Acid Membrane Potentials Divalent Cell membrane Mice chemistry.chemical_compound Potassium Channel Blockers medicine Animals Molecular Biology Fluorescent Dyes chemistry.chemical_classification Lucifer yellow Voltage-gated ion channel Lysine General Neuroscience Tetraethylammonium Neural Inhibition Isoquinolines Taste Buds Electric Stimulation Flufenamic Acid medicine.anatomical_structure Flufenamic acid Gene Expression Regulation chemistry Biochemistry DIDS Neurology (clinical) Ion Channel Gating Developmental Biology medicine.drug |
| Zdroj: | Brain Research. 1373:17-24 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2010.12.019 |
| Popis: | We show here the expression, permeability and pharmacology of a voltage-gated channel in certain taste bud cells (TBCs) which is known to be permeable to Lucifer Yellow CH (LY) and known to release ATP as a neurotransmitter in response to taste substances. LY dissolved in a 200 mM K(+)-containing solution label TBCs immunoreactive to PLCβ2, a phospholipase subtype, but not the TBC subtype immunoreactive to SNAP-25, a SNARE protein. In addition to these subtypes, LY also labelled a few of the non-immunoreactive TBCs. Monovalent and divalent anion probes with molar mass less than 1200 also label PLCβ2-immunoreactive TBCs and a few non-immunoreactive TBCs, whereas a cation probe, rhodamine B, labels the cell membrane of TBCs nonselectively and K(+) independently. The number of LY-labelled TBCs is decreased by 5 μM DIDS (4,4'-diisothiocyanostilbene-2-2'disulfonate), 1mM octanol and 10(-5)M H(+), but not by 10 μM carbenoxolone, 2mM probenecid, 10mM TEA, or 30 μM flufenamic acid. PLCβ2-immunoreactive TBCs and a few non-immunoreactive TBCs generate a TEA-insensitive outwardly rectifying current. DIDS decreases this current in magnitude with IC(50) of ~0.4 μM in a voltage-independent manner. Also 10(-5)M H(+) and 1mM octanol decreases the current magnitude, but 10 μM carbenoxolone and 2mM probenecid do not. These results show that the LY-permeable channel preferably permeates anions and occurs not only on PLCβ2-immunoreactive TBCs but also on certain non-immunoreactive TBCs. Also the results show that the pharmacology of the LY-permeable channel is different from hemichannels reported. The discussion focuses on the pharmacology and the role of the LY-permeable channel. |
| Databáze: | OpenAIRE |
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