Detection of nandrolone, testosterone, and their esters in rat and human hair samples
| Autor: | Chad R. Borges, Robert E. Joseph, Diana G. Wilkins, Douglas E. Rollins, Karin M. Höld |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adult
Male medicine.medical_specialty medicine.drug_class Health Toxicology and Mutagenesis medicine.medical_treatment Toxicology Gas Chromatography-Mass Spectrometry Analytical Chemistry Steroid Anabolic Agents Internal medicine medicine Environmental Chemistry Animals Humans Nandrolone Rats Long-Evans Testosterone Chemical Health and Safety Chromatography integumentary system biology Testosterone acetate Chemistry Pigmentation Reproducibility of Results biology.organism_classification Androgen Rats Substance Abuse Detection Endocrinology Gas chromatography–mass spectrometry Quantitative analysis (chemistry) Cabello medicine.drug Hair |
| Zdroj: | Journal of analytical toxicology. 23(6) |
| ISSN: | 0146-4760 |
| Popis: | Nandrolone and testosterone are anabolic androgenic steroids occasionally abused by athletes. A sensitive, specific, and reproducible gas chromatography-mass spectrometry method for the quantitative determination of nandrolone, testosterone, and their esters in hair has been developed. The limits of quantitation of this method, based on 20 mg of hair, were 50 pg/mg for nandrolone and testosterone, 100 pg/mg for testosterone acetate, and 200 pg/mg for nandrolone-decanoate. Nandrolone-d3 and testosterone-d3 were used as internal standards. This method has been applied to the analysis of these compounds incorporated into rat and human hair. Male Long-Evans rats were given nandrolone decanoate 60 mg/kg intraperitoneally (i.p.) once daily for 10 days over a time period of 14 days. Two of the three rats contained nandrolone in the pigmented hair collected at day 21 at a concentration of 63 and 76 pg/mg, respectively. No drug was found in the corresponding nonpigmented hair. The rat hair samples that tested positive for nandrolone contained also nandrolone decanoate in concentrations of 0.9 and 1.2 ng/mg, respectively. In a separate experiment rats were given testosterone acetate 10 mg/kg i.p. once daily for five days. No testosterone or testosterone acetate was detected in the rat hair samples. Hair specimens were also obtained from four self-reported steroid users. The hair of two subjects were determined to be positive for testosterone in concentrations of 54 and 81 pg/mg. These data demonstrate that it is possible to detect the steroids nandrolone, testosterone, and nandrolone decanoate in hair after systemic administration. |
| Databáze: | OpenAIRE |
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