Oral warfarin affects some aspects of systemic immunomodulation with topical dinitrochlorobenzene (DNCB) in rats

Autor: Dragan Kataranovski, Sandra Belij-Rammerstorfer, Vesna Subota, Ivana Mirkov, Jelena Kulas, Aleksandra Popov Aleksandrov, Milena Kataranovski
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
Neutrophils
Administration
Topical
Administration
Oral
Nitric Oxide
Toxicology
Peripheral blood mononuclear cell
Nitric oxide
Immunomodulation
Leukocyte Count
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Peripheral blood polymorphonuclear cells
Dinitrochlorobenzene
Animals
Medicine
Interleukin 6
Peroxidase
Respiratory Burst
Prothrombin time
medicine.diagnostic_test
biology
Interleukin-6
Tumor Necrosis Factor-alpha
business.industry
Oral warfarin intake
Anticoagulants
General Medicine
Rats
Respiratory burst
Topical dinitrochlorobenzene
030104 developmental biology
chemistry
Peripheral blood mononuclear cells
030220 oncology & carcinogenesis
Myeloperoxidase
Immunology
Prothrombin Time
biology.protein
Partial Thromboplastin Time
Tumor necrosis factor alpha
Warfarin
business
Haptens
Partial thromboplastin time
Zdroj: Cutaneous and Ocular Toxicology
ISSN: 1556-9535
1556-9527
DOI: 10.1080/15569527.2017.1328690
Popis: Purpose: The efficacy of topical dinitrochlorobenzene (DNCB) in the treatment of some skin dermatoses is based both on local and systemic effects. It is not known, however, whether it can be applied to patients receiving some other therapy associated with systemic immunomodulation. The aim of the present paper using a rat model was to examine whether oral warfarin (WF) intake, as shown by others and by us, had an immunomodulatory potential to interfere with effects of topical DNCB as systemic immunotherapy. Materials and methods: Rats received 3.5 mg/l of WF sodium in drinking water for 30 days and were thereafter skin-sensitized with 0.4% DNCB. Changes in the oxidative activity (myeloperoxidase/MPO, reduction of nitroblue tetrazolium/NBT and nitric oxide/NO production) as well as tumor necrosis factor (TNF) production by peripheral blood polymorphonuclear cells (PMN) were measured and compared with PMN from sensitized unexposed to WF rats. Results: WF intake enhanced some aspects of PMN activity (intracellular MPO activity and unstimulated NO production) as well as their responsiveness to exogenous stimulation (NBT reduction and TNF production from sensitized animals). However, WF also decreased PMN responsiveness of NO production to stimulation. WF affected NO and TNF production solely by PMN, as no effect on these activities of peripheral blood mononuclear cells was seen. Conclusion: Having in mind that polymorphonuclear leukocytes are the most abundant cell type in peripheral blood in humans, increase of basic aspects of PMN activity described in the present paper might be relevant for consideration of using WF as therapeutic modality in patients topically treated with DNCB.
Databáze: OpenAIRE
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