A genomic and transcriptomic study on the ddt-resistant Trichoderma hamatum FBL 587. First genetic data into mycoremediation strategies for ddt-polluted sites
| Autor: | Eligio Malusa, Fabiana Russo, Loredana Canfora, Ewa M. Furmanczyk, Małgorzata Tartanus, Oriana Maggi, Anna Maria Persiani, Domenico Davolos, Andrea Ceci |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
bioremediation carbohydrate active enzymes (CAZymes) DDT biodegradation fungal genomics genome-scale RNA-Seq hypocreaceae secondary metabolites siderophores Trichoderma hamatum whole genome sequencing Siderophore animal structures QH301-705.5 Biology Microbiology Transcriptome Bioremediation Virology parasitic diseases Biology (General) Gene reproductive and urinary physiology Whole genome sequencing chemistry.chemical_classification Hypocreaceae organic chemicals Mycoremediation Monooxygenase Enzyme chemistry Biochemistry geographic locations |
| Zdroj: | Microorganisms Volume 9 Issue 8 Microorganisms, Vol 9, Iss 1680, p 1680 (2021) |
| Popis: | Trichoderma hamatum FBL 587 isolated from DDT-contaminated agricultural soils stands out as a remarkable strain with DDT-resistance and the ability to enhance DDT degradation process in soil. Here, whole genome sequencing and RNA-Seq studies for T. hamatum FBL 587 under exposure to DDT were performed. In the 38.9 Mb-genome of T. hamatum FBL 587, 10,944 protein-coding genes were predicted and annotated, including those of relevance to mycoremediation such as production of secondary metabolites and siderophores. The genome-scale transcriptional responses of T. hamatum FBL 587 to DDT exposure showed 1706 upregulated genes, some of which were putatively involved in the cellular translocation and degradation of DDT. With regards to DDT removal capacity, it was found upregulation of metabolizing enzymes such as P450s, and potentially of downstream DDT-transforming enzymes such as epoxide hydrolases, FAD-dependent monooxygenases, glycosyl- and glutathione-transferases. Based on transcriptional responses, the DDT degradation pathway could include transmembrane transporters of DDT, antioxidant enzymes for oxidative stress due to DDT exposure, as well as lipases and biosurfactants for the enhanced solubility of DDT. Our study provides the first genomic and transcriptomic data on T. hamatum FBL 587 under exposure to DDT, which are a base for a better understanding of mycoremediation strategies for DDT-polluted sites. |
| Databáze: | OpenAIRE |
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