An LC-MRM method for measuring intestinal triglyceride assembly using an oral stable isotope-labeled fat challenge
| Autor: | Achilles Alon, Justin Dennie, Omar F Laterza, Michael E. Lassman, Thomas McAvoy, David G. McLaren, Derek L Chappell, Sudha S. Shankar, Helene L. Cardasis, Gino Salituro, Manu Chakravarthy, Xiaofang Li, Jennifer E Lambert, Elizabeth J. Parks |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Adolescent Enterocyte Clinical Biochemistry Absorption (skin) 030204 cardiovascular system & hematology Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Young Adult 0302 clinical medicine medicine Humans General Pharmacology Toxicology and Pharmaceutics Intestinal Mucosa Chromatography High Pressure Liquid Triglycerides chemistry.chemical_classification Triglyceride Stable isotope ratio Fatty acid General Medicine Deuterium Diet Medical Laboratory Technology 030104 developmental biology medicine.anatomical_structure chemistry Biochemistry Isotope Labeling Composition (visual arts) Intracellular Oleic Acid |
| Zdroj: | Bioanalysis. 8(12) |
| ISSN: | 1757-6199 |
| Popis: | Aim: A traditional oral fatty acid challenge assesses absorption of triacylglycerol (TG) into the periphery through the intestines, but cannot distinguish the composition or source of fatty acid in the TG. Stable isotope-labeled tracers combined with LC-MRM can be used to identify and distinguish TG synthesized with dietary and stored fatty acids. Results: Concentrations of three abundant TGs (52:2, 54:3 and 54:4) were monitored for incorporation of one or two 2H11-oleate molecules per TG. This method was subjected to routine assay validation and meets typical requirements for an assay to be used to support clinical studies. Conclusion: Calculations for the fractional appearance rate of TG in plasma are presented along with the intracellular enterocyte precursor pool for 12 study participants. |
| Databáze: | OpenAIRE |
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