Aligned nanofibers of decellularized muscle extracellular matrix for volumetric muscle loss
Autor: | Andrew J. Dunn, Muhamed Talovic, Sara Vendrell, Koyal Garg, Mark Schwartz, Krishna Patel, Anjali Patel |
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Rok vydání: | 2019 |
Předmět: |
Muscle tissue
Male Scaffold Materials science Polyesters Biomedical Engineering Nanofibers Muscle Proteins 02 engineering and technology Muscle Development Biomaterials Extracellular matrix 03 medical and health sciences Mice medicine Myocyte Animals Regeneration Muscle Strength Muscle Skeletal 030304 developmental biology 0303 health sciences Decellularization Arginase Tissue Scaffolds Regeneration (biology) Macrophages Stem Cells technology industry and agriculture Skeletal muscle Organ Size 021001 nanoscience & nanotechnology Extracellular Matrix Mice Inbred C57BL medicine.anatomical_structure 0210 nano-technology Biomedical engineering Reinnervation |
Zdroj: | Journal of biomedical materials research. Part B, Applied biomaterialsREFERENCES. 108(6) |
ISSN: | 1552-4981 |
Popis: | Volumetric muscle loss (VML) is a traumatic loss of muscle tissue that results in chronic functional impairment. When injured, skeletal muscle is capable of small-scale repair; however, regenerative capacities are lost with VML due to a critical loss stem cells and extracellular matrix (ECM). Consequences of VML include either long-term disability or delayed amputations of the affected limb. While the prevalence of VML is substantial, currently a successful clinical therapy has not been identified. In a previous study, an electrospun composed of polycaprolactone (PCL) and decellularized-ECM (D-ECM) supported satellite cell-mediated myogenic activity in vitro. In this study, we investigate the extent to which this electrospun scaffold can support functional muscle regeneration in a murine model of VML. Experimental groups included no treatment, pure PCL treated, and PCL:D-ECM (50:50 blend) treated VML defects. The PCL:D-ECM scaffold treated VML muscles supported increased activity of anti-inflammatory M2 macrophages (arginase+ ) at Day 28, compared to other experimental groups. Increased myofiber (MHC+ ) regeneration was observed histologically at both Days 7 and 28 post-trauma in blend scaffold treated group compared to PCL treated and untreated groups. However, improvements in muscle weights and force production were not observed. Future studies would evaluate muscle function at longer time-points post-VML injury to allow sufficient time for reinnervation of regenerated muscle fibers. |
Databáze: | OpenAIRE |
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