Interaction of silver nanoparticles with algae and fish cells: a side by side comparison
| Autor: | Marc J.-F. Suter, Yang Yue, Smitha Pillai, Xiaomei Li, Renata Behra, Kristin Schirmer, Laura Sigg |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Gills
Euglena gracilis ved/biology.organism_classification_rank.species Cell Culture Techniques Metal Nanoparticles Medicine (miscellaneous) Pharmaceutical Science 02 engineering and technology 010501 environmental sciences 01 natural sciences Applied Microbiology and Biotechnology Silver nanoparticle Nanoparticle-protein interactions Nanoparticle toxicity Nanoparticle uptake biology 021001 nanoscience & nanotechnology Endocytosis Oncorhynchus mykiss Toxicity Molecular Medicine Sodium-Potassium-Exchanging ATPase 0210 nano-technology Intracellular Fish Proteins Silver RTgill-W1 cell line Biomedical Engineering Nanotechnology Bioengineering Cell Line Cell wall Algae Microscopy Electron Transmission Extracellular Animals Particle Size 0105 earth and related environmental sciences AgNP ved/biology Research biology.organism_classification Alkaline Phosphatase Culture Media Cell culture Biophysics Adsorption Water Pollutants Chemical |
| Zdroj: | Journal of Nanobiotechnology Journal of Nanobiotechnology, 15 (1) |
| ISSN: | 1477-3155 |
| DOI: | 10.1186/s12951-017-0254-9 |
| Popis: | Background Silver nanoparticles (AgNP) are widely applied and can, upon use, be released into the aquatic environment. This raises concerns about potential impacts of AgNP on aquatic organisms. We here present a side by side comparison of the interaction of AgNP with two contrasting cell types: algal cells, using the algae Euglena gracilis as model, and fish cells, a cell line originating from rainbow trout (Oncorhynchus mykiss) gill (RTgill-W1). The comparison is based on the AgNP behavior in exposure media, toxicity, uptake and interaction with proteins. Results (1) The composition of exposure media affected AgNP behavior and toxicity to algae and fish cells. (2) The toxicity of AgNP to algae was mediated by dissolved silver while nanoparticle specific effects in addition to dissolved silver contributed to the toxicity of AgNP to fish cells. (3) AgNP did not enter into algal cells; they only adsorbed onto the cell surface. In contrast, AgNP were taken up by fish cells via endocytic pathways. (4) AgNP can bind to both extracellular and intracellular proteins and inhibit enzyme activity. Conclusion Our results showed that fish cells take up AgNP in contrast to algal cells, where AgNP sorbed onto the cell surface, which indicates that the cell wall of algae is a barrier to particle uptake. This particle behaviour results in different responses to AgNP exposure in algae and fish cells. Yet, proteins from both cell types can be affected by AgNP exposure: for algae, extracellular proteins secreted from cells for, e.g., nutrient acquisition. For fish cells, intracellular and/or membrane-bound proteins, such as the Na+/K+-ATPase, are susceptible to AgNP binding and functional impairment. Journal of Nanobiotechnology, 15 (1) ISSN:1477-3155 |
| Databáze: | OpenAIRE |
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