17alpha-ethyl-5beta-estrane-3alpha, 17beta-diol, a biological marker for the abuse of norethandrolone and ethylestrenol in slaughter cattle

Autor: R.F.M. Maas, A. S. J. P. A. M. van Miert, Renger F. Witkamp, L. Hendriks, J. Raus, M.-P. Jacobs, M. Van Puymbroeck, M.E.M. Kuilman, Peter Adriaensens, L. Leyssens, D. Vanderzande
Přispěvatelé: Centraal Instituut voor Voedingsonderzoek TNO
Rok vydání: 1999
Předmět:
Male
Magnetic Resonance Spectroscopy
Metabolite
Urine
Drug residue
Thin layer chromatography
Animal husbandry
Animal tissue
chemistry.chemical_compound
Feces
Ultraviolet spectrophotometry
Animal Husbandry
Biotransformation
Liver cell culture
Chromatography
High Pressure Liquid
Priority journal
Ethylestrenol
Estradiol
Norethandrolone
Reference Standards
Drug monitoring
Standard
Chemistry
Liver
Estrane
Microsomes
Liver
Steroids
Biological Markers
Female
Liver microsome
17α-Ethyl-5β-estrane-3α
17β-diol
medicine.drug
17Alpha ethyl 5 xi estrane 3 xi
17 beta diol
Drug derivative
Mass fragmentography
Drug abuse
Sensitivity and Specificity
Article
Gas Chromatography-Mass Spectrometry
medicine
Animals
Animal experiment
Nuclear magnetic resonance spectroscopy
Nutrition
Reduction
Drug metabolism
Chromatography
Animal
General Chemistry
Metabolism
Nonhuman
Drug Residues
Biological marker
chemistry
Microsome
Cattle
Spectrophotometry
Ultraviolet
Chromatography
Thin Layer
Intramuscular drug administration
Biomarkers
High performance liquid chromatography
Zdroj: Journal of Chromatography B: Biomedical Sciences and Applications, 2, 728, 217-232
ISSN: 1387-2273
Popis: The metabolism of the illegal growth promoter ethylestrenol (EES) was evaluated in bovine liver cells and subcellular fractions of bovine liver preparations. Incubations with bovine microsomal preparations revealed that EES is extensively biotransformed into norethandrolone (NE), another illegal growth promoter. Furthermore, incubations of monolayer cultures of hepatocytes with NE indicated that NE itself is rapidly reduced to 17α-ethyl-5β-estrane-3α,17β-diol (EED). In vivo tests confirmed that, after administration of either EES or NE, EED is excreted as a major metabolite. Therefore, it was concluded that, both in urine and faeces samples, EED can be used as a biological marker for the illegal use of EES and/or NE. Moreover, by monitoring EED in urine or faeces samples, the detection period after NE administration is significantly prolonged. These findings were further confirmed by three cases of norethandrolone abuse in a routine screening program for forbidden growth promoters. Copyright (C) 1999 Elsevier Science B.V.
Databáze: OpenAIRE
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