Delivery of Methylene Blue and meso-tetra (N-methyl-4-pyridyl) porphine tetra tosylate from cross-linked poly(vinyl alcohol) hydrogels: A potential means of photodynamic therapy of infected wounds
| Autor: | Paul A. McCarron, Corona M. Cassidy, Mark G. Jenkins, Ryan G Loughlin, Anthony Brown, Michael M. Tunney, Ryan F. Donnelly |
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| Rok vydání: | 2009 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
Serum Vinyl alcohol Porphyrins Light medicine.medical_treatment Biophysics Photodynamic therapy medicine.disease_cause Microbiology chemistry.chemical_compound Drug Delivery Systems In vivo medicine Animals Humans Radiology Nuclear Medicine and imaging Photosensitizing Agents Radiation Radiological and Ultrasound Technology Hydrogels Methylene Blue Animals Newborn Photochemotherapy chemistry Staphylococcus aureus Polyvinyl Alcohol Self-healing hydrogels Drug delivery Wound Infection Cattle Phototoxicity Methylene blue Nuclear chemistry |
| Zdroj: | Journal of Photochemistry and Photobiology B: Biology. 96:223-231 |
| ISSN: | 1011-1344 |
| DOI: | 10.1016/j.jphotobiol.2009.06.010 |
| Popis: | Poly(vinyl alcohol)-borate complexes were evaluated as a potentially novel drug delivery platform suitable for in vivo use in photodynamic antimicrobial chemotherapy (PACT) of wound infections. An optimised formulation (8.0%w/w PVA, 2.0% w/w borax) was loaded with 1.0 mg ml(-1) of the photosensitisers Methylene Blue (MB) and meso-tetra (N-methyl-4-pyridyl) porphine tetra tosylate (TMP). Both drugs were released to yield receiver compartment concentrations (>5.0 mu g ml(-1)) found to be phototoxic to both planktonic and bicifilm-grown methicillin-resistant Staphylococcus aureus (MRSA), a common cause of wound infections in hospitals. Newborn calf serum, used to simulate the conditions prevalent in an exuding wound, did not adversely affect the properties of the hydrogels and had no significant effect on the rate of TMP-mediated photodynamic kill of MRSA, despite appreciably reducing the fluence rate of incident light. However, MB-mediated photodynamic kill of MRSA was significantly reduced in the presence of calf serum and when the clinical isolate was grown in a biofilm. Results support the contention that delivery of MB or TMP using gel-type vehicles as part of PACT could make a contribution to the photodynamic eradication of MRSA from infected wounds. (C) 2009 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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