Folic acid-functionalized gadolinium-loaded phase transition nanodroplets for dual-modal ultrasound/magnetic resonance imaging of hepatocellular carcinoma
| Autor: | Seyed Hossein Hejazi, Roghayeh Kamran Samani, Fatemeh Maghsoudinia, Masoud A. Mehrgardi, Tayebe Sobhani, Fatemeh Mehradnia, Mohamad Bagher Tavakoli |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Carcinoma
Hepatocellular Gadolinium Contrast Media chemistry.chemical_element 02 engineering and technology 01 natural sciences Analytical Chemistry Mice Folic Acid Nuclear magnetic resonance In vivo medicine Animals medicine.diagnostic_test business.industry Liver Neoplasms 010401 analytical chemistry Ultrasound Cancer Magnetic resonance imaging 021001 nanoscience & nanotechnology medicine.disease Magnetic Resonance Imaging 0104 chemical sciences chemistry Hepatocellular carcinoma Cancer cell Nanoparticles Molecular imaging 0210 nano-technology business |
| Zdroj: | Talanta. 228:122245 |
| ISSN: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2021.122245 |
| Popis: | Dual-modal molecular imaging by combining two imaging techniques can provide complementary information for early cancer diagnosis and therapeutic monitoring. In the present manuscript, folic acid (FA)-functionalized gadolinium-loaded nanodroplets (NDs) are introduced as dual-modal ultrasound (US)/magnetic resonance (MR) imaging contrast agents. These phase-change contrast agents (PCCAs) with alginate (Alg) stabilizing shell and a liquid perfluorohexane (PFH) core were successfully synthesized via the nano-emulsion method and characterized. In this regard, mouse hepatocellular carcinoma (Hepa1-6) as target cancer cells and mouse fibroblast (L929) as control cells were used. The in vitro and in vivo cytotoxicity assessments indicated that Gd/PFH@Alg and Gd/PFH@Alg-FA nanodroplets are highly biocompatible. Gd-loaded NDs do not induce organ toxicity, and no significant hemolytic activity in human red blood cells is observed. Additionally, nanodroplets exhibited strong ultrasound signal intensities as well as T1-weighted MRI signal enhancement with a high relaxivity value of 6.40 mM−1 s−1, which is significantly higher than that of the clinical Gadovist contrast agent (r1 = 4.01 mM−1 s−1). Cellular uptake of Gd-NDs-FA by Hepa1-6 cancer cells was approximately 2.5-fold higher than that of Gd-NDs after 12 h incubation. Furthermore, in vivo results confirmed that the Gd-NDs-FA bound selectively to cancer cells and were accumulated in the tumor region. In conclusion, Gd/PFH@Alg-FA nanodroplets have great potential as US/MR dual-modal imaging nanoprobes for the early diagnosis of cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect