Application of microfluidic chip with integrated optics for electrophoretic separations of proteins

Autor: Benjamin Hannes, Stanislas Krawczyk, Radoslaw Mazurczyk, C.J. Morin, Yann Chevolot, P.L. Desbène, Julien Vieillard
Přispěvatelé: Laboratoire d'électronique, optoélectronique et microsystèmes (LEOM), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), LASOC (LASOC), Sciences et Méthodes Séparatives (SMS), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)
Rok vydání: 2006
Předmět:
Optics and Photonics
Fabrication
Capillary action
Clinical Biochemistry
Microfluidics
[CHIM.THER]Chemical Sciences/Medicinal Chemistry
02 engineering and technology
01 natural sciences
Biochemistry
Analytical Chemistry
law.invention
Capillary electrophoresis
[CHIM.ANAL]Chemical Sciences/Analytical chemistry
law
System on a chip
Chromatography
Lab-on-a-chip
[CHIM.ORGA]Chemical Sciences/Organic chemistry
Chemistry
010401 analytical chemistry
technology
industry
and agriculture
Electrophoresis
Capillary
Proteins
Reproducibility of Results
Integrated optics
[CHIM.CATA]Chemical Sciences/Catalysis
Cell Biology
General Medicine
Gel electrophoresis of proteins
Carbocyanines
Microfluidic Analytical Techniques
021001 nanoscience & nanotechnology
0104 chemical sciences
[CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry
Electrophoresis
Microfluidic
Streptavidin
Electroosmosis
0210 nano-technology
[CHIM.CHEM]Chemical Sciences/Cheminformatics
Zdroj: Journal of Chromatography B Biomedical Sciences and Applications
Journal of Chromatography B Biomedical Sciences and Applications, Elsevier, 2007, 845 (2), pp.218-225. ⟨10.1016/j.jchromb.2006.08.009⟩
ISSN: 1570-0232
0378-4347
DOI: 10.1016/j.jchromb.2006.08.009⟩
Popis: International audience; This paper describes the fabrication, the characterization and the applications of a capillary electrophoresis microchip. This hybrid device (glass/PDMS) features channels and optical waveguides integrated in one common substrate. It can be used for electrophoretic separation and fluorimetric detection of molecules. The microfluidic performance of the device is demonstrated by capillary zone and gel electrophoresis of proteins.
Databáze: OpenAIRE
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