Effect of qi-tonifying and stasis-eliminating therapy on expression of vascular endothelial growth factor and its receptors Flt-1, Flk-1 in the brain of intracerebral hemorrhagic rats
| Autor: | Tao Tang, Ding-zhu Shen, Ju-Fang Huang, Yong Qi, Can-jie Guo, Zhihua Xing, Zong-Qi Zhang, Yi-qiang Jin, Hua-xian Zhang, Qi-Dong Yang, Jiekun Luo, Xing-Qun Li |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A medicine.medical_specialty Qi Rats Sprague-Dawley chemistry.chemical_compound Internal medicine Forelimb medicine Animals Pharmacology (medical) Medicine Chinese Traditional Receptor Cerebral Hemorrhage Intracerebral hemorrhage Vascular Endothelial Growth Factor Receptor-1 Behavior Animal business.industry Brain General Medicine medicine.disease Vascular Endothelial Growth Factor Receptor-2 Normal group Rats Vascular endothelial growth factor medicine.anatomical_structure Endocrinology Complementary and alternative medicine chemistry Buyang huanwu decoction Collagenase Immunohistochemistry Female business medicine.drug Phytotherapy |
| Zdroj: | Chinese journal of integrative medicine. 13(4) |
| ISSN: | 1672-0415 |
| Popis: | To investigate the effects and mechanism of qi-tonifying and stasis-eliminating (QTSE) therapy on the expression of vascular endothelial growth factor (VEGF) and its receptors Flt-1 and Flk-1 in the brains of intracerebral hemorrhagic (model) rats. One hundred and eighty Sprague-Dawley rats were randomly divided into six groups: the normal group (n=5), the sham-operative (SO) group (n=35), the model group (n=35), the QTSE group (n=35), the QT group (n=35) and the SE group (n=35). All the rats except those in the normal group and SO group were established into an intracerebral hemorrhage(ICH) model by intracerebral injection of collagenase type VII and the latter three were orally administered with Buyang Huanwu Decoction (补阳还五汤, a classical recipe for QTSE) or with some of its components for qi-tonification and for stasis-elimination, respectively. To the other three groups, normal saline solutions were given instead. Behavioral tests were carried out in the animals randomly chosen from each group on days 1, 2, 4, 7, 14, 21 and 28 after modeling. The expressions of VEGF, Flk-1 and Flt-1 were determined by immunohistochemistry and the number of vascular segments with positive expression in the injured brain area of the rats was calculated. From day 7 onwards, the asymmetric forelimb use rate in the QTSE group recovered more significantly than that in the other model groups. In the model group, the expressions of VEGF, Flk-1 and Flt-1 appeared on day 1 and reached a peak on day 21, then weakened gradually. In the QTSE group, as compared with the other model groups, a higher level of VEGF expression was shown from day 7 (P |
| Databáze: | OpenAIRE |
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