Invadopodia are required for cancer cell extravasation and are a therapeutic target for metastasis
| Autor: | Konstantin Stoletov, Sara A. Courtneidge, Ann F. Chambers, Hon S. Leong, Victor A. McPherson, M. Nicole Hague, Andrew E. Chien, Curtis A. Chin, Sean J. Leith, Carl O. Postenka, Amber Ablack, John D. Lewis, Eva A. Turley, Amy E. Robertson, Katia Carmine-Simmen |
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| Rok vydání: | 2013 |
| Předmět: |
Lung Neoplasms
Invadopodium Mice Nude Antineoplastic Agents Chick Embryo Biology General Biochemistry Genetics and Molecular Biology Metastasis 03 medical and health sciences Mice 0302 clinical medicine Stroma Transcellular Cell Migration Cell Line Tumor medicine Matrix Metalloproteinase 14 Animals Humans Benzodioxoles Neoplasm Metastasis lcsh:QH301-705.5 Protein Kinase Inhibitors 030304 developmental biology Adaptor Proteins Signal Transducing 0303 health sciences Anatomy Phosphate-Binding Proteins medicine.disease Phosphoproteins Extravasation lcsh:Biology (General) 030220 oncology & carcinogenesis Cancer cell Invadopodia Cancer research biology.protein Neoplastic Stem Cells Quinazolines Female Cell Surface Extensions Cortactin |
| Zdroj: | Cell Reports, Vol 8, Iss 5, Pp 1558-1570 (2014) |
| ISSN: | 2211-1247 |
| Popis: | Summary Tumor cell extravasation is a key step during cancer metastasis, yet the precise mechanisms that regulate this dynamic process are unclear. We utilized a high-resolution time-lapse intravital imaging approach to visualize the dynamics of cancer cell extravasation in vivo. During intravascular migration, cancer cells form protrusive structures identified as invadopodia by their enrichment of MT1-MMP, cortactin, Tks4, and importantly Tks5, which localizes exclusively to invadopodia. Cancer cells extend invadopodia through the endothelium into the extravascular stroma prior to their extravasation at endothelial junctions. Genetic or pharmacological inhibition of invadopodia initiation (cortactin), maturation (Tks5), or function (Tks4) resulted in an abrogation of cancer cell extravasation and metastatic colony formation in an experimental mouse lung metastasis model. This provides direct evidence of a functional role for invadopodia during cancer cell extravasation and distant metastasis and reveals an opportunity for therapeutic intervention in this clinically important process. |
| Databáze: | OpenAIRE |
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