Comparative study on characterization and wound healing potential of goat (Capra hircus) mesenchymal stem cells derived from fetal origin amniotic fluid and adult bone marrow
Autor: | Anjali Somal, Indu Baiju, Vikash Chandra, Amar Nath, G. Saikumar, Irfan A. Bhatt, G. Taru Sharma, Nitin E. Gade, Amarpal, Pawan K. Dubey, M.D. Pratheesh, Sreekumar Tr, Bhaskar Sharma, V.L. Gleeja, T. B. Sivanarayanan, D. N. Madhu |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Cell type Pathology medicine.medical_specialty Amniotic fluid Bone Marrow Cells Biology Neovascularization Random Allocation 03 medical and health sciences medicine Animals Wound Healing integumentary system General Veterinary Goats Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Amniotic stem cells Amniotic Fluid 030104 developmental biology medicine.anatomical_structure Wounds and Injuries Collagen Rabbits Bone marrow Stem cell medicine.symptom Wound healing Stem Cell Transplantation |
Zdroj: | Research in Veterinary Science. 112:81-88 |
ISSN: | 0034-5288 |
DOI: | 10.1016/j.rvsc.2016.12.009 |
Popis: | Caprine amniotic fluid (cAF) and bone marrow cells (cBM) were isolated, expanded and phenotypically characterized by mesenchymal stem cells (MSCs) specific cell surface markers. Both cell types were compared for multilineage differentiation potential by flow cytometry using specific antibodies against lineage specific markers. Furthermore, in vitro expanded cAF-MSCs showed higher expression of trophic factors viz. VEGF and TGF-β1 as compared to cBM-MSCs. Full-skin thickness excisional wounds created on either side of the dorsal midline (thoracolumbar) of New Zealand White rabbits were randomly assigned to subcutaneous injection of either fetal origin cAF-MSCs (n=4) or adult cBM-MSCs (n=4) or sterile PBS (control, n=4). The rate of wound closure was found faster (p |
Databáze: | OpenAIRE |
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