Continuous, on-line DNA sequencing using a versatile infrared laser scanner/electrophoresis apparatus
| Autor: | John A. Brumbaugh, Scott L. Sutter, Gabor Patonay, Gary D. Sloniker, David L. Steffens, Robert C. Bruce, John C. Bruce, Daniel L. Grone, Robert D. Eckles, Stephen C. Roemer, Lyle Richard Middendorf |
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| Rok vydání: | 1992 |
| Předmět: |
Fluorophore
Microscope Spectrophotometry Infrared Infrared Clinical Biochemistry Molecular Sequence Data Analytical chemistry DNA Single-Stranded Biochemistry DNA sequencing Analytical Chemistry law.invention chemistry.chemical_compound law Fluorescence microscope Image Processing Computer-Assisted Gel electrophoresis Laser diode Base Sequence Molecular Structure business.industry Lasers Far-infrared laser Signal Processing Computer-Assisted DNA Templates Genetic chemistry Microscopy Fluorescence Optoelectronics Electrophoresis Polyacrylamide Gel business |
| Zdroj: | Electrophoresis. 13(8) |
| ISSN: | 0173-0835 |
| Popis: | A new apparatus for continuously detecting fluorescently labeled DNA fragments is based on infrared fluorescence technology. This technology combines state-of-the-art developments in chemistry, laser technology, and detection, while achieving improved reliability, sensitivity, and flexibility for applications including DNA sequencing. DNA molecules labeled with a novel infrared fluorophore are detected during electrophoresis using a scanning infrared fluorescence microscope. The microscope consists of a laser diode for exciting the fluorophore and a silicon avalanche photodiode for detecting the infrared emission. Optimum conditions for detection and throughput are obtained by adjusting electrophoresis, scanning and imaging parameters. Typical DNA sequencing runs (test templates) allow identification of over 500 bases per sample with greater than 99% accuracy. |
| Databáze: | OpenAIRE |
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