Role for exosomes with self-antigens and immune regulatory molecules in allo- and auto-immunity leading to chronic immune injury following murine kidney transplantation

Autor: Yoshihiro Itabashi, Ranjithkumar Ravichandran, Sandhya Bansal, Chiahsuan Chin, Christin Poulson, Angara Sureshbabu, Sumi Sukumaran Nair, Sudhir Perincheri, T. Mohanakumar
Rok vydání: 2022
Předmět:
Zdroj: Transplant Immunology. 75:101702
ISSN: 0966-3274
DOI: 10.1016/j.trim.2022.101702
Popis: Antibodies against donor human leukocyte antigen are a risk factor for chronic immune injury (CII) following renal transplantation; however, it is often not detectable. The main goal of this study is to gain new insights into the kinetics of exosome release and content in sensitized vs non-sensitized recipients. Towards this, we investigated the role for circulating exosomes with allo and self-antigens as well as immunoregulatory molecules in the development of CII and acute rejection.Using murine kidney allograft rejection models, we investigated the role of exosomes on immune responses leading to allo- and auto-immunity to self-antigens resulting in rejection. Exosomes were analyzed for kidney self-antigens (Collagen-IV, fibronectin, angiotensin II receptor type 1), and immune-regulatory molecules (PD-L1, CD73) using western blot. Antibodies to donor MHC in serum samples were detected by immunofluorescence, self-antigens by enzyme-linked immunosorbent assay and kidney tissue infiltrating cells were determined by immunohistochemistry.BALB/c; H2We demonstrate a pivotal role for induction of exosomes with immune-regulatory molecules, allo- and auto-immunity to self-antigens leading to chronic immune injury following murine kidney transplantation.
Databáze: OpenAIRE
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