In vitro antifungal activity and cytotoxicity screening of dry crude extracts from Brazilian Amazonia plants
| Autor: | Maria das Graças Afonso Miranda Chaves, Marcela Mota, Mônica Regina Pereira Senra Soares, César Augusto Caneschi, Pedro Henrique Fazza Stroppa, Nádia Rezende Barbosa Raposo, Wagner Luiz Ramos Barbosa |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
biology Candida glabrata Traditional medicine biology.organism_classification Antimicrobial In vitro Fungicide Multiple drug resistance 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Complementary and alternative medicine 030220 oncology & carcinogenesis Candida krusei Drug Discovery Candidiasis Natural products Toxicity Candida albicans Cytotoxicity |
| Zdroj: | African Journal of Traditional, Complementary and Alternative Medicines; Vol 15, No 4 (2018); 13-21 |
| ISSN: | 0189-6016 |
| Popis: | Background: Antifungal multidrug resistance has been reported worldwide and has stimulated investigations of plant species for the treatment of candidiasis. In particular, in vitro antifungal activities and cytotoxicity of dry extracts from Ceasalpinia ferrea (tul.) Martius, Brosimum acutifolium Huber, and Salacia impressifolia (Miers) A.C. Smith were evaluated. Materials and Methods: Minimum inhibitory concentrations (MIC) and minimum fungicide (MFC) values were established according to the protocol M27-A2 of the Clinical and Laboratory Standards Institute (CLSI). Subsequent evaluations were performed using strains of Candida albicans from the American Type Culture Collection (ATCC) 10231, clinical isolated Candida albicans, Candida glabrata (CCT) 0728, Candida krusei (FTI) CCT 1517, and Candida guilliermondii (CCT) 1890. Morphological changes were evaluated using scanning electron microscopy (SEM), and cytotoxicity was evaluated in murine L929 fibroblast cells after treatment with plant extracts. Results: MIC values indicated antifungal potential of all three extracts against the main fungi that causes candidiasis. Conclusion: In particular, C. ferrea showed promising antimicrobial potential against all strains. Hence, future studies are warranted to investigate pharmacologically active compounds from this extract that could be used as prototypes for drug development and/or as a source of raw pharmaceutical materials for the treatment of candidiasis. |
| Databáze: | OpenAIRE |
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