Anti-biofilm and anti-inflammatory effects of Lycosin-II isolated from spiders against multi-drug resistant bacteria
| Autor: | Yoonkyung Park, Jonggwan Park, Jun Hee Oh |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
Erythrocytes Lipopolysaccharide medicine.drug_class Multi drug resistant bacteria Antibiotics Biophysics Anti-Inflammatory Agents Spider Venoms medicine.disease_cause Gram-Positive Bacteria Biochemistry Microbiology chemistry.chemical_compound Phagocytosis Drug Resistance Multiple Bacterial Gram-Negative Bacteria medicine Animals Humans Sheep biology Pseudomonas aeruginosa Spiders Cell Biology Fibroblasts biology.organism_classification Antimicrobial Anti-Bacterial Agents chemistry Staphylococcus aureus Biofilms Cytokines Lipoteichoic acid Bacteria Antimicrobial Peptides |
| Zdroj: | Biochimica et biophysica acta. Biomembranes. 1864(1) |
| ISSN: | 1879-2642 |
| Popis: | Currently, multidrug-resistant bacteria are rapidly increasing worldwide because of the misuse or overuse of antibiotics. In particular, few options exist for treating infections caused by long-persisting oxacillin-resistant strains and recently proliferating carbapenem-resistant strains. Therefore, alternative treatments are urgently needed. The antimicrobial peptide (AMP) Lycosin-II is a peptide consisting of 21 amino acids isolated from the venom of the spider Lycosa singoriensis. Lycosin-II showed strong antibacterial activity and biofilm inhibition effects against gram-positive and gram-negative bacteria including oxacillin-resistant Staphylococcus aureus (S. aureus) and meropenem-resistant Pseudomonas aeruginosa (P. aeruginosa) isolated from patients. In addition, Lycosin-II was not cytotoxic against human foreskin fibroblast Hs27 or hemolytic against sheep red blood cells at the concentration of which exerted antibacterial activity. The mechanism of action of Lycosin-II involves binding to lipoteichoic acid and lipopolysaccharide of gram-positive and gram-negative bacterial membranes, respectively, to destroy the bacterial membrane. Moreover, Lycosin-II showed anti-inflammatory effects by inhibiting the expression of pro-inflammatory cytokines that are increased during bacterial infection in Hs27 cells. These results suggest that Lycosin-II can serve as a therapeutic agent against infections with multidrug-resistant strains. |
| Databáze: | OpenAIRE |
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