c-Jun promotes neurite outgrowth and survival in PC12 cells
| Autor: | Alexander Muravlev, Mike Dragunow, Hannah M. Gibbons, P. Lawlor, Deborah Young, Ruian Xu, Andree G. Pearson, Janusz Lipski, Ann-Marie Woodgate, Matthew J. During, G.A. MacGibbon, M. Walton |
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| Rok vydání: | 2000 |
| Předmět: |
Programmed cell death
Patch-Clamp Techniques Neurite Cell Survival Proto-Oncogene Proteins c-jun Action Potentials Apoptosis Biology Transfection PC12 Cells Sodium Channels Cellular and Molecular Neuroscience Genes Reporter Nerve Growth Factor Okadaic Acid Neurites Animals Enzyme Inhibitors Phosphorylation Luciferases Molecular Biology Neurons Regulation of gene expression c-jun Cell Differentiation Molecular biology Rats Transcription Factor AP-1 Phenotype Cell culture |
| Zdroj: | Molecular Brain Research. 83:20-33 |
| ISSN: | 0169-328X |
| DOI: | 10.1016/s0169-328x(00)00191-1 |
| Popis: | We investigated the function of c-Jun in PC12 cells by transfecting them with a plasmid containing a c-Jun cDNA transcription cassette. Transfected cells expressed high levels of c-Jun mRNA and protein and demonstrated an increase in both AP-1 DNA binding and gene activation. The c-Jun over-expressing cells showed marked neurite outgrowth but no evidence of spontaneous cell death. In fact, c-Jun over-expressing cells were more resistant to okadaic acid-induced apoptosis. The process outgrowth was not indicative of a full neuronal differentiation response as the transfected PC12 cells did not display action potentials when examined with whole-cell patch-clamping. The phosphorylation of c-Jun on serine 73 appears to be important for this neurite sprouting effect as mutagenesis at this site reduced sprouting whereas a serine 63 mutant tended to increase sprouting. Thus, in PC12 cells c-Jun expression does not induce apoptosis, but rather functions as a neurite outgrowth and neuronal survival signal. |
| Databáze: | OpenAIRE |
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