Analysis of Paramecium Macronuclear DNA Using Pulsed Field Gel Electrophoresis
| Autor: | James D. Forney, Elizabeth H. Blackburn, Huan L. Phan |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Electrophoresis
Agar Gel Gel electrophoresis Paramecium biology Macronucleus Nucleic Acid Hybridization biology.organism_classification Ribosome Molecular biology Blotting Southern Nucleic acid thermodynamics chemistry.chemical_compound chemistry RNA Ribosomal Animals Nucleic Acid Conformation Parasitology Paramecium tetraurelia DNA Circular Ribosomal DNA DNA |
| Zdroj: | The Journal of Protozoology. 36:402-408 |
| ISSN: | 0022-3921 |
| Popis: | We have analyzed the macronuclear DNA of Paramecium tetraurelia using orthogonal-field-alternation gel electrophoresis. The mean size of the linear macronuclear DNA molecules is approximately 450 kb. Less than 6% of the macronuclear DNA is larger than 800 kb. Using pulse times of 20, 40, 60 and 90 s we show that the macronuclear fragment containing the A type variable surface antigen gene migrates reproducibly as a 320-kb linear DNA. Over the same pulse times we describe the unusual migration of the ribosomal RNA gene (rDNA) of P. tetraurelia. At pulse times of 20 and 40 s the rDNA migrates at limit mobility (300 and 500 kb, respectively) whereas with 60- and 90-s pulse times, 2 components of rDNA are observed; 1 fraction independent of pulse time migrating at limit mobility, and a 2nd component migrating between 100-kb and 400-kb linear markers. Based upon previous electron micrographic studies of Paramecium rDNA as well as data presented here we conclude that the majority of Paramecium rDNA molecules are a circular DNA form. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text