DNA barcoding and surveillance sampling strategies for Culicoides biting midges (Diptera: Ceratopogonidae) in southern India

Autor: Yella Narasimha Reddy, Swathi Laban, Naveen Kumar, Lara E. Harrup, Glenn A Bellis, Bruno Mathieu, Rien De Keyser, Kondappa Muniramaiah Purushotham, David Gopurenko, Shrikant Kowalli, Karien Labuschagne, Yarabolu Krishnamohan Reddy, Gaya Prasad, Bethan V. Purse, Claire Garros, S. M. Byregowda, James G. Logan, Simon Carpenter, Minakshi Prasad, Alison A. Bettis
Přispěvatelé: Harrup, Lara E., Vector-borne Viral Diseases Programme, The Pirbright Institute, Tamil Nadu Veterinary and Animal Sciences University, Natural Environment Research Council (NERC), College of Veterinary Science, Acharya N.G. Ranga Agricultural University, Institute of Animal Health and Veterinary Biologicals (IAHVB), Anhui Agriculture University (AHAU), University of Veterinary and Animal Sciences, Indian Council of Agricultural Research (ICAR), Department of Disease Control, London School of Hygiene and Tropical Medicine (LSHTM), Contrôle des maladies animales exotiques et émergentes (UMR CMAEE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Wagga Wagga Agricultural Institute (WWAI), New South Wales Department of Primary Industries (NSW DPI), EH Graham Centre for Agricultural Innovation, Department of Agriculture, Fisheries and Forestry ( DAFF ), Onderstepoort Veterinary Institute (ARC - OVI), Department of Zoology and Entomology [Pretoria], University of Pretoria [South Africa], Institut de Parasitologie et de Pathologie Tropicale (IPPTS), This study was funded by the Biotechnology and Biological Sciences Research Council (BBSRC), Department for International Development (DFID) and Scottish Government grant BB/H009205/1, BB/H009167/1 and BB/H009493/1 as part of the Combating Infectious Diseases of Livestock for International Development initiative. With additional support provided by BBSRC grant BB/K021214/1, AB was also supported with funding from the London School of Hygiene and Tropical Medicine. The Indian Council of Agricultural Research supported this study through its Hisar, Chennai, Bengaluru and Hyderabad centres under the All Indian Network Programme on Bluetongue.
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Entomology
Veterinary medicine
Identification
Phylogénie
Biodiversité et Ecologie
ADN
led
Ceratopogonidae
L73 - Maladies des animaux
DNA barcoding
0302 clinical medicine
Génétique des populations
culicoides
bluetongue virus
arbovirus
DNA barcode
bold
coi
surveillance
Phylogeny
biology
Culicoides
Infectious Diseases
Vecteur de maladie
PCR
Population Surveillance
Fièvre catarrhale du mouton
Empreinte ADN
L72 - Organismes nuisibles des animaux
identification morphologique
Species complex
code barre adn
030231 tropical medicine
India
Biodiversity and Ecology
03 medical and health sciences
Species Specificity
Animals
DNA Barcoding
Taxonomic
Surveillance épidémiologique
Technique analytique
fièvre catarrhale ovine
Research
Outbreak
DNA
biology.organism_classification
arboviruses [EN]
inde
diversité des populations
030104 developmental biology
Biology and Microbiology
Parasitology
Vector (epidemiology)
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
Animal Distribution
Zdroj: Parasites and Vectors (9), 20 p.. (2016)
Parasites and Vectors
Parasites and Vectors, BioMed Central, 2016, 9, 20 p. ⟨10.1186/s13071-016-1722-z⟩
Parasites & Vectors
ISSN: 1756-3305
DOI: 10.1186/s13071-016-1722-z⟩
Popis: Background Culicoides spp. biting midges transmit bluetongue virus (BTV), the aetiological agent of bluetongue (BT), an economically important disease of ruminants. In southern India, hyperendemic outbreaks of BT exert high cost to subsistence farmers in the region, impacting on sheep production. Effective Culicoides spp. monitoring methods coupled with accurate species identification can accelerate responses for minimising BT outbreaks. Here, we assessed the utility of sampling methods and DNA barcoding for detection and identification of Culicoides spp. in southern India, in order to provide an informed basis for future monitoring of their populations in the region. Methods Culicoides spp. collected from Tamil Nadu and Karnataka were used to construct a framework for future morphological identification in surveillance, based on sequence comparison of the DNA barcode region of the mitochondrial cytochrome c oxidase I (COI) gene and achieving quality standards defined by the Barcode of Life initiative. Pairwise catches of Culicoides spp. were compared in diversity and abundance between green (570 nm) and ultraviolet (UV) (390 nm) light emitting diode (LED) suction traps at a single site in Chennai, Tamil Nadu over 20 nights of sampling in November 2013. Results DNA barcode sequences of Culicoides spp. were mostly congruent both with existing DNA barcode data from other countries and with morphological identification of major vector species. However, sequence differences symptomatic of cryptic species diversity were present in some groups which require further investigation. While the diversity of species collected by the UV LED Center for Disease Control (CDC) trap did not significantly vary from that collected by the green LED CDC trap, the UV CDC significantly outperformed the green LED CDC trap with regard to the number of Culicoides individuals collected. Conclusions Morphological identification of the majority of potential vector species of Culicoides spp. samples within southern India appears relatively robust; however, potential cryptic species diversity was present in some groups requiring further investigation. The UV LED CDC trap is recommended for surveillance of Culicoides in southern India. Electronic supplementary material The online version of this article (doi:10.1186/s13071-016-1722-z) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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