Chronological Change of Vascular Reactivity to cGMP Generators in the Balloon-Injured Rat Carotid Artery

Autor: Matomo Nishio, Takashi Shimosato, Takayoshi Masuoka, Tomio Okamura, Takaharu Ishibashi, Masashi Tawa, Hiroshi Sakonjo
Rok vydání: 2019
Předmět:
Male
inorganic chemicals
0301 basic medicine
medicine.medical_specialty
Time Factors
Hydrocarbons
Fluorinated
Physiology
Carotid arteries
Sodium
Enzyme Activators
chemistry.chemical_element
030204 cardiovascular system & hematology
Balloon
Benzoates
Second Messenger Systems
Muscle
Smooth
Vascular
Nitric oxide
Rats
Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
Soluble Guanylyl Cyclase
0302 clinical medicine
Internal medicine
medicine
Animals
Nitric Oxide Donors
heterocyclic compounds
Sodium nitrite
Cyclic GMP
Sodium Nitrite
business.industry
Biphenyl Compounds
medicine.disease
Enzyme Activation
Vasodilation
Disease Models
Animal
Stenosis
Carotid Arteries
030104 developmental biology
medicine.anatomical_structure
Endocrinology
chemistry
cardiovascular system
Immunohistochemistry
Carotid Artery Injuries
Cardiology and Cardiovascular Medicine
business
Angioplasty
Balloon
Artery
Zdroj: Journal of Vascular Research. 56:109-116
ISSN: 1423-0135
1018-1172
DOI: 10.1159/000498896
Popis: Background/Aims: Soluble guanylate cyclase (sGC) exists as reduced, oxidized, and heme-free forms. Currently, it is unclear whether endovascular mechanical stenosis has an impact on vascular tone control by drugs targeting sGC, namely cGMP generators. Methods: Pharmacological responses to acidified sodium nitrite (reduced sGC stimulant) and BAY 60-2770 (oxidized/heme-free sGC stimulant) were studied in balloon-injured rat carotid arteries at several time points. In addition, sGC expression was detected by immunohistochemistry. Results: At 1 day after injury, acidified sodium nitrite-induced relaxation was attenuated in the injured artery, whereas BAY 60-2770-induced relaxation was augmented. Similar attenuation of response to acidified sodium nitrite was seen at 7 and 14 days after injury. On the other hand, the augmentation of response to BAY 60-2770 disappeared at 7 and 14 days after injury. At 1 day after injury, the immunohistochemical expression pattern of sGC in the smooth muscle layer of the injured artery was not different from that of the uninjured artery. However, in the injured artery, the intensity of sGC staining was weak at 7 and 14 days after injury. Conclusion: Balloon injury alters vascular responsiveness to cGMP generators, which seems to be associated with the form and/or expression of sGC.
Databáze: OpenAIRE
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