CTRP3 promotes TNF-α-induced apoptosis and barrier dysfunction in salivary epithelial cells
| Autor: | Ling-Han Qu, Jia-Zeng Su, Xin Cong, Yan Zhang, Guang-Yan Yu, Li-Ling Wu, Ruo-Lan Xiang, Mei Mei |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Ductal cells Submandibular Gland Apoptosis Caspase 3 Caspase 8 Diabetes Mellitus Experimental Mice 03 medical and health sciences 0302 clinical medicine Adipokines stomatognathic system medicine Animals Humans FADD biology Salivary gland Tumor Necrosis Factor-alpha Chemistry Epithelial Cells Cell Biology Molecular biology Submandibular gland Rats 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Tumor Necrosis Factors biology.protein Tumor necrosis factor alpha |
| Zdroj: | Cellular Signalling. 85:110042 |
| ISSN: | 0898-6568 |
| DOI: | 10.1016/j.cellsig.2021.110042 |
| Popis: | Background C1q/tumour necrosis factor-related protein 3 (CTRP3) plays important roles in metabolism and inflammatory responses in various cells and tissues. However, the expression and function of CTRP3 in salivary glands have not been explored. Methods The expression and distribution of CTRP3 were detected by western blot, polymerase chain reaction, immunohistochemical and immunofluorescence staining. The effects of CTRP3 on tumour necrosis factor (TNF)-α-induced apoptosis and barrier dysfunction were detected by flow cytometry, western blot, co-immunoprecipitation, and measurement of transepithelial resistance and paracellular tracer flux. Results CTRP3 was distributed in both acinar and ductal cells of human submandibular gland (SMG) and was primarily located in the ducts of rat and mouse SMGs. TNF-α increased the apoptotic rate, elevated expression of cleaved caspase 3 and cytochrome C, and reduced B cell lymphoma-2 (Bcl-2) levels in cultured human SMG tissue and SMG-C6 cells, and CTRP3 further enhanced TNF-α-induced apoptosis response. Additionally, CTRP3 aggravated TNF-α-increased paracellular permeability. Mechanistically, CTRP3 promoted TNF-α-enhanced TNF type I receptor (TNFR1) expression, inhibited the expression of cellular Fas-associated death domain (FADD)-like interleukin-1β converting enzyme inhibitory protein (c-FLIP), and increased the recruitment of FADD with receptor-interacting protein kinase 1 and caspase 8. Moreover, CTRP3 was significantly increased in the labial gland of Sjogren's syndrome patients and in the serum and SMG of nonobese diabetic mice. Conclusions These findings suggest that the salivary glands are a novel source of CTRP3 synthesis and secretion. CTRP3 might promote TNF-α-induced cell apoptosis through the TNFR1-mediated complex II pathway. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect