Micropatterning of endothelial cells to create a capillary-like network with defined architecture by laser-assisted bioprinting
| Autor: | Jean-Michel Bourget, Sylvie Crauste-Manciet, Murielle Rémy, Olivia Kérourédan, Sylvain Catros, Jérôme Kalisky, Raphaël Devillard, Noélie B. Thebaud |
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| Přispěvatelé: | Chassande, Olivier, Bioingénierie tissulaire (BIOTIS), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM), Pôle d’Odontologie et de Santé Buccale, CHU Bordeaux [Bordeaux], Energie, matériaux et télécommunication, Institut National de la Recherche Scientifique [Québec] (INRS), Acides Nucléiques : Régulations Naturelle et Artificielle (ARNA), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Vascular Endothelial Growth Factor A
Materials science Capillary action [SDV]Life Sciences [q-bio] 0206 medical engineering Biomedical Engineering Biophysics Biocompatible Materials Bioengineering 02 engineering and technology Cell Line Biomaterials Mice chemistry.chemical_compound Human Umbilical Vein Endothelial Cells Animals Humans Tissue Engineering Tissue Scaffolds Lasers Mesenchymal stem cell Bioprinting Infant Newborn Endothelial Cells Biomaterial Hydrogels 021001 nanoscience & nanotechnology Laser assisted Molar 020601 biomedical engineering Coculture Techniques Vascular endothelial growth factor [SDV] Life Sciences [q-bio] chemistry Cell culture Printing Three-Dimensional Collagen 0210 nano-technology Biomedical engineering Biofabrication Micropatterning |
| Zdroj: | Journal of Materials Science: Materials in Medicine Journal of Materials Science: Materials in Medicine, 2019, 30 (2), pp.28. ⟨10.1007/s10856-019-6230-1⟩ Journal of Materials Science: Materials in Medicine, Springer Verlag, 2019, 30 (2), pp.28. ⟨10.1007/s10856-019-6230-1⟩ |
| ISSN: | 0957-4530 1573-4838 |
| DOI: | 10.1007/s10856-019-6230-1⟩ |
| Popis: | International audience; Development of a microvasculature into tissue-engineered bone substitutes represents a current challenge. Seeding of endothelial cells in an appropriate environment can give rise to a capillary-like network to enhance prevascularization of bone substitutes. Advances in biofabrication techniques, such as bioprinting, could allow to precisely define a pattern of endothelial cells onto a biomaterial suitable for in vivo applications. The aim of this study was to produce a microvascular network following a defined pattern and preserve it while preparing the surface to print another layer of endothelial cells. We first optimise the bioink cell concentration and laser printing parameters and then develop a method to allow endothelial cells to survive between two collagen layers. Laser-assisted bioprinting (LAB) was used to pattern lines of tdTomato-labeled endothelial cells cocultured with mesenchymal stem cells seeded onto a collagen hydrogel. Formation of capillary-like structures was dependent on a sufficient local density of endothelial cells. Overlay of the pattern with collagen I hydrogel containing vascular endothelial growth factor (VEGF) allowed capillary-like structures formation and preservation of the printed pattern over time. Results indicate that laser-assisted bioprinting is a valuable technique to pre-organize endothelial cells into high cell density pattern in order to create a vascular network with defined architecture in tissue-engineered constructs based on collagen hydrogel. |
| Databáze: | OpenAIRE |
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