Development of a SCAR (sequence characterised amplified region) marker for molecular tagging of the dwarf BREIZH (Bzh) gene in Brassica napus L
| Autor: | Régine Delourme, Michel Renard, N. Foisset, Pierre Barret |
|---|---|
| Přispěvatelé: | UMR 0118 UMR INRA / ENSAR : Génétique et amélioration des plantes, Institut National de la Recherche Agronomique (INRA)-Génétique et amélioration des plantes (G.A.P.)-UMR INRA / ENSAR : Génétique et amélioration des plantes (RENN UMR GENET AMELIOR PLANTES), ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
0106 biological sciences
Genetics 0303 health sciences [SDV.GEN]Life Sciences [q-bio]/Genetics Nucleic acid sequence food and beverages Locus (genetics) [SDV.GEN] Life Sciences [q-bio]/Genetics General Medicine Marker-assisted selection Biology 01 natural sciences Homology (biology) RAPD 03 medical and health sciences Restriction enzyme Genetic marker Agronomy and Crop Science Gene COLZA ComputingMilieux_MISCELLANEOUS 030304 developmental biology 010606 plant biology & botany Biotechnology |
| Zdroj: | TAG Theoretical and Applied Genetics TAG Theoretical and Applied Genetics, Springer Verlag, 1998, 97, pp.828-833 |
| ISSN: | 0040-5752 1432-2242 |
| Popis: | A SCAR (sequence characterised amplified region) has been developed for optimal tagging of the dwarf Bzh gene in Brassica napus. A RAPD marker named OPMO7-730 was previously found closely linked to the dwarf locus at 0.8±0.7 cM. The DNA band corresponding to this marker was cloned and sequenced, and specific PCR primers were designed. The PCR test allowed us to amplify the locus corresponding to OPM07-730. With a restriction endonuclease digest and optimal electrophoresis conditions, three allelic forms of this marker have been recovered on the 40 B. napus accessions tested. The usefullness of this marker in breeding dwarf rapeseed lines is discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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