Establishment of TUSMi003-A, an induced pluripotent stem cell (iPSC) line from a 62-year old Chinese Han patient with Alzheimer's disease with ApoE3/4 genetic background
| Autor: | Gang Li, Shulin Meng, Ying Wang, Keni Chen, Ying Lei, Jing Zhang, Jian Zhao, Hongxiang Yu, Yue Zhang, Heng Sun |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genotype Immunocytochemistry Apolipoprotein E4 Induced Pluripotent Stem Cells Karyotype Apolipoprotein E3 Disease Germ layer Biology Peripheral blood mononuclear cell 03 medical and health sciences Asian People Alzheimer Disease Humans Induced pluripotent stem cell Transcription factor lcsh:QH301-705.5 Cell Biology General Medicine Middle Aged Immunohistochemistry 030104 developmental biology lcsh:Biology (General) Immunology Leukocytes Mononuclear Female Biomarkers Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 27, Iss, Pp 57-60 (2018) |
| ISSN: | 1876-7753 |
| Popis: | A 62-year old Chinese Han Alzheimer's disease (AD) female patient with ApoE3/4 genetic background donated her Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system was used to reprogrammed PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in AD. |
| Databáze: | OpenAIRE |
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