In vivo characterization of functional states of cortical microglia during peripheral inflammation
| Autor: | Michael T. Heneka, Daria Savitska, Elizabeta Zirdum, Bianca Brawek, Nicole Fröhlich, Nima Mojtahedi, Olga Garaschuk, Karin Riester |
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| Rok vydání: | 2020 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine Immunology Motility Inflammation 03 medical and health sciences Behavioral Neuroscience 0302 clinical medicine ddc:150 medicine Humans Central element Innate immune system Microglia Endocrine and Autonomic Systems Chemistry Effector Brain Phenotype 030104 developmental biology medicine.anatomical_structure medicine.symptom Neuroscience 030217 neurology & neurosurgery Intracellular Signal Transduction |
| Zdroj: | Brain, behavior and immunity 87, 243-255 (2020). doi:10.1016/j.bbi.2019.12.007 |
| ISSN: | 0889-1591 |
| Popis: | Peripheral inflammation is known to trigger a mirror inflammatory response in the brain, involving brain's innate immune cells - microglia. However, the functional phenotypes, which these cells adopt in the course of peripheral inflammation, remain obscure. In vivo two-photon imaging of microglial Ca2+ signaling as well as process motility reveals two distinct functional states of cortical microglia during a lipopolysaccharide-induced peripheral inflammation: an early 'sensor state' characterized by dramatically increased intracellular Ca2+ signaling but ramified morphology and a later 'effector state' characterized by slow normalization of intracellular Ca2+ signaling but hypertrophic morphology, substantial IL-1β production in a subset of cells as well as increased velocity of directed process extension and loss of coordination between individual processes. Thus, lipopolysaccharide-induced microglial Ca2+ signaling might represent the central element connecting receptive and executive functions of microglia. |
| Databáze: | OpenAIRE |
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