Combining next-generation sequencing and single-molecule sequencing to explore brown plant hopper responses to contrasting genotypes of japonica rice
| Autor: | Rongzhi Chen, Chaomei Huang, Lili Zhu, Jing Zhang, Bo Du, Cong Zhou, Yu Chen, Danax Huanhan, Guangcun He, Wei Guan, Yinxia Hu, Jianping Guo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
lcsh:QH426-470 Genotype lcsh:Biotechnology Autophagy-Related Proteins RNA-Seq Biology urologic and male genital diseases 01 natural sciences DNA sequencing Transcriptome Fusion gene Hemiptera 03 medical and health sciences lcsh:TP248.13-248.65 Genetics Plant defense against herbivory Animals Herbivory Gene Transaminases 030304 developmental biology Glutathione Transferase 0303 health sciences Caspase 8 Oryza sativa Forkhead Box Protein O1 food and beverages Oryza Catalase lcsh:Genetics Host resistance Brown plant hopper Gene Expression Regulation Ferritins Female RNA Long Noncoding Single-molecule real-time sequencing 010606 plant biology & botany Biotechnology Single molecule real time sequencing Research Article FoxO signaling pathway Signal Transduction |
| Zdroj: | BMC Genomics BMC Genomics, Vol 20, Iss 1, Pp 1-18 (2019) |
| ISSN: | 1471-2164 |
| Popis: | Background The brown plant hopper (BPH), Nilaparvata lugens, is one of the major pest of rice (Oryza sativa). Plant defenses against insect herbivores have been extensively studied, but our understanding of insect responses to host plants’ resistance mechanisms is still limited. The purpose of this study is to characterize transcripts of BPH and reveal the responses of BPH insects to resistant rice at transcription level by using the advanced molecular techniques, the next-generation sequencing (NGS) and the single-molecule, real-time (SMRT) sequencing. Results The current study obtained 24,891 collapsed isoforms of full-length transcripts, and 20,662 were mapped to known annotated genes, including 17,175 novel transcripts. The current study also identified 915 fusion genes, 1794 novel genes, 2435 long non-coding RNAs (lncRNAs), and 20,356 alternative splicing events. Moreover, analysis of differentially expressed genes (DEGs) revealed that genes involved in metabolic and cell proliferation processes were significantly enriched in up-regulated and down-regulated sets, respectively, in BPH fed on resistant rice relative to BPH fed on susceptible wild type rice. Furthermore, the FoxO signaling pathway was involved and genes related to BPH starvation response (Nlbmm), apoptosis and autophagy (caspase 8, ATG13, BNIP3 and IAP), active oxygen elimination (catalase, MSR, ferritin) and detoxification (GST, CarE) were up-regulated in BPH responses to resistant rice. Conclusions The current study provides the first demonstrations of the full diversity and complexity of the BPH transcriptome, and indicates that BPH responses to rice resistance, might be related to starvation stress responses, nutrient transformation, oxidative decomposition, and detoxification. The current result findings will facilitate further exploration of molecular mechanisms of interaction between BPH insects and host rice. Electronic supplementary material The online version of this article (10.1186/s12864-019-6049-7) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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