Cloning and sequence analysis of the phosphoprotein gene of rinderpest virus
| Autor: | Melkote S. Shaila, Thomas Barrett, M. D. Baron |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Whole genome sequencing
Genetics Base Sequence Genes Viral Sequence Homology Amino Acid Sequence analysis Molecular Sequence Data Nucleic acid sequence Biology Rinderpest virus Phosphoproteins Virology Molecular biology Homology (biology) Primer extension Viral Proteins Measles virus Complementary DNA Paramyxoviridae RNA Viral Amino Acid Sequence Cloning Molecular Gene |
| Zdroj: | The Journal of general virology. 74 |
| ISSN: | 0022-1317 |
| Popis: | We have cloned several cDNAs derived from the P gene of rinderpest virus. One of these, derived from a bicistronic N-P mRNA, has been sequenced in its entirety. Sequencing of a section of the others, and comparison with the genome sequence, showed that P gene transcripts, as for other morbilliviruses, were variable; non-templated Gs could be added at a site resembling the normal stop transcription site. Primer extension analysis showed that about half the transcripts were edited. Sequences of the P, C and V proteins encoded by the normal and edited transcripts were compared with those of other morbilliviruses and with those of the more distantly related paramyxoviruses. |
| Databáze: | OpenAIRE |
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