Lipid production in Porphyridium cruentum grown under different culture conditions
| Autor: | Do-Hyung Kang, Myoung Hoon Jeong, Hyeon Yong Lee, Shin Young Lee, Sung Ho Oh, Seung Seop Kim, Young Mi Kim, Ji Hye Ha, Na-Young Kim, Jeong Sub Cho, Won Byong Yoon, Hyang Suk Jeong, Jae Gun Han |
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| Rok vydání: | 2009 |
| Předmět: |
Biodiesel
biology Cell Survival Cell growth Cell Culture Techniques Heterotroph Bioengineering Lipid Metabolism biology.organism_classification Applied Microbiology and Biotechnology chemistry.chemical_compound Bioreactors Species Specificity Porphyridium cruentum chemistry Biofuels Biodiesel production Botany Glycerol Autotroph Food science Porphyridium Mixotroph Cell Proliferation Biotechnology |
| Zdroj: | Journal of Bioscience and Bioengineering. 108:429-434 |
| ISSN: | 1389-1723 |
| Popis: | Autotrophic growth of Porphyridium cruentum under 18:12 h and 12:12 h light:dark cycles showed the maximum cell concentration of 2.1 g-dry wt./L, whereas the specific growth rate, 0.042 (1/h), at 18:6 h is faster than that of 12:12 h, 0.031 (1/h), respectively. The highest lipid accumulation level, 19.3 (%, w/w), was achieved at 12:12 h cycle. Under dark cultivation condition with 10 g/L of glucose, the lipid accumulation in the cell was 10.9 (%, w/w), whereas the heterotrophic growth with glycerol as the carbon resource showed low level of cell concentration and lipid production, compared to that of glucose. The glucose was decided to be a suitable carbon resource for the heterotrophic growth of P. cruentum. The lipids from P. cruentum seemed be feasible for biodiesel production, because over 30% of the lipid was C16-C(18:1). The cultivation time and temperature were important factors to increase the maximum cell concentration. Extending the cultivation time helps maintain the maximum cell concentration, and higher lipid accumulation was achieved at 25 degrees C, compared to 35 degrees C. The fed-batch cultures showed that, under the light condition, the specific production rate was slightly decreased to 0.4% lipid/g-dry wt./day at the later stage, whereas, under the dark condition, the specific production rate was maintained to be a maximum value of 1.1% lipid/g-dry wt./day, even in the later stage of cultivation. The results indicate that the heterotrophic or 12:12 h cyclic mixotrophic growth of P. cruentum could be used for the production of biodiesel in long-term fed-batch cultivation of P. cruentum. |
| Databáze: | OpenAIRE |
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