Use of high-performance liquid chromatography for preparing samples for microsequencing
| Autor: | May Chang, Stanley Stein, Russell Blacher, Yu-Ching E. Pan, Janusz Wideman |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Chromatography
Edman degradation Sequence analysis Chemistry Hydrolysis Organic Chemistry food and beverages General Medicine Biochemistry High-performance liquid chromatography Analytical Chemistry Interferon Type I Humans Trypsin Amino Acid Sequence Cyanogen Bromide Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography A. 297:13-19 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(01)89024-5 |
| Popis: | A method has been developed for the microsequencing of protein at subnanomole levels. The protein is carboxymethylated and freed of salts and reagents by reversed-phase chromatography prior to automated Edman degradation on a gas-phase sequencer. The carboxymethylated protein can also be fragmented chemically or enzymatically for further sequence analysis. The analytical techniques used to monitor the progress of the reactions all have picomole level sensitivity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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