Fast uncoiling kinetics of F1C pili expressed by uropathogenic Escherichia coli are revealed on a single pilus level using force-measuring optical tweezers
| Autor: | Bernt Eric Uhlin, Stina Lindberg, Sarah Ehlers, Mickaël Castelain, Jeanna Klinth, Magnus Andersson, Ove Axner |
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| Přispěvatelé: | Department of physics, Umeå University, Department of Molecular Biology, Swedish Research Council 621-2008-3280 2007-3574, Kempe foundations, Magnus Bergvall's foundation |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Protein Folding
Optical Tweezers Fimbria Microscopy Atomic Force urologic and male genital diseases medicine.disease_cause Fimbriae Bacterial/*chemistry/classification Pilus Protein Structure Secondary Uropathogenic Escherichia coli/*chemistry/physiology/*ultrastructure Uropathogenic Escherichia coli Microscopy Atomic Force/methods Biomechanics 0303 health sciences Chemistry General Medicine Adhesion female genital diseases and pregnancy complications Biomechanical Phenomena dynamic force spectroscopy Optical tweezers Pili relaxation Urinary Tract Infections Bond kinetics Protein folding uncoiling Fimbriae Proteins [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] Kinetics Static Electricity Biophysics Bacterial Adhesion/*physiology Fimbriae Proteins/analysis/chemistry Models Biological Microbiology 03 medical and health sciences Organelle medicine Escherichia coli 030304 developmental biology Urinary Tract Infections/microbiology 030306 microbiology biochemical phenomena metabolism and nutrition bacterial infections and mycoses bacterial adhesion Fimbriae Bacterial bacteria Stress Mechanical |
| Zdroj: | European Biophysics Journal European Biophysics Journal, Springer Verlag (Germany), 2011, 40 (3), pp.305-316. ⟨10.1007/s00249-010-0648-1⟩ |
| ISSN: | 0175-7571 1432-1017 |
| DOI: | 10.1007/s00249-010-0648-1⟩ |
| Popis: | International audience; Uropathogenic Escherichia coli (UPEC) express various kinds of organelles, so-called pili or fimbriae, that mediate adhesion to host tissue in the urinary tract through specific receptor-adhesin interactions. The biomechanical properties of these pili have been considered important for the ability of bacteria to withstand shear forces from rinsing urine flows. Force-measuring optical tweezers have been used to characterize individual organelles of F1C type expressed by UPEC bacteria with respect to such properties. Qualitatively, the force-versus-elongation response was found to be similar to that of other types of helix-like pili expressed by UPEC, i.e., type 1, P, and S, with force-induced elongation in three regions, one of which represents the important uncoiling mechanism of the helix-like quaternary structure. Quantitatively, the steady-state uncoiling force was assessed as 26.4 +/-1.4 pN, which is similar to those of other pili (which range from 21 pN for S(I) to 30 pN for type 1). The corner velocity for dynamic response (1,400 nm/s) was found to be larger than those of the other pili (400-700 nm/s for S and P pili, and 6 nm/s for type 1). The kinetics were found to be faster, with a thermal opening rate of 17 Hz, a few times higher than S and P pili, and three orders of magnitude higher than type 1. These data suggest that F1C pili are, like P and S pili, evolutionarily selected to primarily withstand the conditions expressed in the upper urinary tract. |
| Databáze: | OpenAIRE |
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