Intercellular cell adhesion molecule-1 regulates lymphocyte movement into intestinal microlymphatics of rat Peyer's patches

Autor: Hiroshi Nagata, D. Neil Granger, Soichiro Miura, Seiichiro Koseki, Hitoshi Fujimori, Hiromasa Ishii, Ryota Hokari, Iwao Kurose, Shingo Kato, Eiichi Sekizuka
Rok vydání: 2001
Předmět:
Zdroj: Journal of Leukocyte Biology. 70:896-902
ISSN: 1938-3673
0741-5400
DOI: 10.1189/jlb.70.6.896
Popis: The objective of this study was to determine whether specific adhesion molecules modulate lymphocyte movement from Peyer's patches into intestinal microlymphatics. The fluorochrome acridine orange was injected via a micropipette into Peyer's patches to fill lymphatics. The flux of labeled lymphocytes into intestinal microlymphatics was monitored with intravital fluorescence microscopy. The lymphatic microvessels in the perifollicular area of Peyer's patches were filled with lymphocytes, most of which remained within the lymphatics. Some lymphocytes became detached and were drained into intestinal lymph. Administration of antibodies directed against ICAM-1 significantly increased lymphocyte flux into interfollicular lymphatics. The immunohistochemical study showed intense ICAM-1 expression on the lymphocytes densely packed in the lymphatics surrounding follicles in Peyer's patches. A large number of lymphocytes are normally sequestered in the lymphatic network of Peyer's patches. This sequestration of lymphocytes is largely mediated by ICAM-1-dependent cell-cell interactions.
Databáze: OpenAIRE
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