The role of DJ-1/Nrf2 pathway in the pathogenesis of diabetic nephropathy in rats
| Autor: | Hui-zhang Liu, Wei-na Duan, Qing-Tao Meng, Zi-ying Shen, Qian Sun, Zhongyuan Xia |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty NF-E2-Related Factor 2 medicine.medical_treatment Protein Deglycase DJ-1 Intraperitoneal injection Critical Care and Intensive Care Medicine medicine.disease_cause Rats Sprague-Dawley Pathogenesis Diabetic nephropathy 03 medical and health sciences chemistry.chemical_compound Diabetes mellitus Internal medicine Animals Medicine Diabetic Nephropathies biology business.industry General Medicine medicine.disease Streptozotocin Malondialdehyde Rats 030104 developmental biology Endocrinology Cystatin C chemistry Nephrology biology.protein business Oxidative stress Signal Transduction medicine.drug |
| Zdroj: | Renal Failure. 38:294-304 |
| ISSN: | 1525-6049 0886-022X |
| DOI: | 10.3109/0886022x.2015.1120119 |
| Popis: | Diabetic nephropathy (DN) is one of the most common chronic complications of diabetes, which is associated with an increased oxidative stress induced by hyperglycemia and alterations in DJ-1/NF-E2-related factor-2 (Nrf2) pathway. In the present study, we investigated the role and the proper time nodes of DJ-1/Nrf2 pathway in the pathogenesis of DN. Diabetes mellitus (DM) model of rats was induced by intraperitoneal injection of streptozotocin (STZ) on male Sprague-Dawley (SD) rats. Then, the diabetic rats were divided into 4, 8 and 12 weeks groups. As early at 4 weeks of diabetes, renal histologic evaluation score, cystatin C (Cys C), β2-microglobulin (β2-MG) and malondialdehyde (MDA) levels were increased, and total antioxidative capacity (T-AOC) level was decreased as compared with that in the control group. The protein expressions of DJ-1, NF-E2-related factor-2 (Nrf2) and heme oxygenase-1 (HO-1) were upregulated compared with the control group from 4 weeks and further increased with the progression of DM. The protein expressions of DJ-1, Nrf2 and HO-1 in renal tissues have good line correlations with renal histologic evaluation score, respectively. Taken together, these results suggested that the activation of DJ-1/Nrf2 pathway was involved in the pathogenesis of diabetic nephropathy in rats. |
| Databáze: | OpenAIRE |
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