The isolation of the gamma subunit of fetal hemoglocin (HbF) and its use in a radioimmunoassay for HbF
| Autor: | J. Shuster, R.F.L. James, Phil Gold, S. O. Freedman |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Male
Antigenicity Immunogen Immunology Radioimmunoassay Cross Reactions Binding Competitive Absorption Antibody Specificity Pregnancy hemic and lymphatic diseases Fetal hemoglobin Immunology and Allergy Animals Humans Globin Fetal Hemoglobin Antiserum Chemistry Immune Sera Molecular biology Abnormal hemoglobin Globins Biochemistry Electrophoresis Polyacrylamide Gel Female Hemoglobin Rabbits |
| Zdroj: | Journal of immunological methods. 32(3) |
| ISSN: | 0022-1759 |
| Popis: | A method is described for the purification, from fetal hemoglobin (HbF), of the fetal specific globin chain (gamma chain) in its native state. In the absence of alpha chain (the globin chain common to all adult human hemoglobins) gamma chain, when used as an immunogen, is able to express its unique antigenicity. Here, a specific, high titer antiserum raised against gamma chain has been used to establish a sensitive radioimmunoassay for HbF. This approach may be applicable to the measurement of other normal and abnormal hemoglobins. |
| Databáze: | OpenAIRE |
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