Sensitivity of Shiga toxin-producing Escherichia coli (STEC) strains for colicins under different experimental conditions
| Autor: | Len J.A. Lipman, Khalil Boutaga, Bart J.A.M. Jordi, Frans van Knapen, Caroline M.E. van Heeswijk |
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| Rok vydání: | 2001 |
| Předmět: |
Serotype
Rumen Colicins Microbial Sensitivity Tests Escherichia coli O157 Shiga Toxins medicine.disease_cause Microbiology fluids and secretions Escherichia coli Genetics medicine Animals Humans Anaerobiosis Serotyping Molecular Biology biology Toxin Shiga toxin biochemical phenomena metabolism and nutrition biology.organism_classification Enterobacteriaceae Aerobiosis Culture Media Colicin biology.protein bacteria Cattle Bacteria |
| Zdroj: | FEMS Microbiology Letters. 204:329-334 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.2001.tb10906.x |
| Popis: | Twenty Escherichia coli strains producing well-characterised colicins were tested for their inhibitory activity against five Shiga toxin-producing E. coli (STEC) strains using different media under aerobic and anaerobic conditions. The five STEC strains used were of serotype O26, O111, O128, O145 and O157:H7 which are frequently isolated serotypes associated with disease in humans. The main route of infection for humans is through the eating of badly cooked or handled beef. The major reservoir for STEC strains in cattle is the rumen. To mimic the situation in the rumen of cattle, overlay assays were also performed under anaerobic conditions in the presence of 30% rumen fluid. Colicins E1, E4, E8-J, K and S4 are most active against STEC strains under anaerobic conditions in the absence or presence of rumen fluid. These colicins will be used in future experiments with the aim to eradicate the presence of STEC in cattle. |
| Databáze: | OpenAIRE |
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