Expression of vascular endothelial growth factor does not promote transformation but confers a growth advantage in vivo to Chinese hamster ovary cells
| Autor: | M. W. Siegel, A D Levinson, A M Rowland, T Burton, Heidi S. Phillips, Gilbert-Andre Keller, T Terrell, Jane Winer, Napoleone Ferrara |
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| Rok vydání: | 1993 |
| Předmět: |
Vascular Endothelial Growth Factor A
medicine.medical_specialty Time Factors medicine.medical_treatment Genetic Vectors Transplantation Heterologous Hamster Mice Nude Enzyme-Linked Immunosorbent Assay CHO Cells Endothelial Growth Factors Biology Transfection chemistry.chemical_compound Mice In vivo Internal medicine Cricetinae medicine Animals Humans RNA Messenger In Situ Hybridization Lymphokines Vascular Endothelial Growth Factors Chinese hamster ovary cell Growth factor General Medicine Neoplasms Experimental Recombinant Proteins Cell biology Clone Cells Vascular endothelial growth factor Vascular endothelial growth factor A Kinetics Endocrinology Cell Transformation Neoplastic chemistry Cell culture Cell Division Neoplasm Transplantation Research Article |
| Zdroj: | The Journal of clinical investigation. 91(1) |
| ISSN: | 0021-9738 |
| Popis: | Vascular endothelial growth factor (VEGF) is a mitogen with a specificity for endothelial cells in vitro and an angiogenic inducer in vivo. We tested the hypothesis that VEGF may confer on expressing cells a growth advantage in vivo. Dihydrofolatereductase--Chinese hamster ovary cells were transfected with expression vectors which direct the constitutive synthesis of VEGF. Neither the expression nor the exogenous administration of VEGF stimulated anchorage-dependent or anchorage-independent growth of Chinese hamster ovary cells in vitro. However, VEGF-expressing clones, unlike control cells, demonstrated an ability to proliferate in nude mice. Histologic examination revealed that the proliferative lesions were compact, well vascularized, and nonedematous. Ultrastructural analysis revealed that capillaries within the lesions were of the continuous type. These findings indicate that the expression of VEGF may confer on cells the ability to grow in vivo in the absence of transformation by purely paracrine mechanisms. Since VEGF is a widely distributed protein, this property may have relevance for a variety of physiological and pathological proliferative processes. |
| Databáze: | OpenAIRE |
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