An early and massive wave of germinal cell apoptosis is required for the development of functional spermatogenesis

Autor: Christiane Ody, Pierre Vassalli, Irène Garcia, Kimi Araki, Ivan Rodriguez
Jazyk: angličtina
Rok vydání: 1997
Předmět:
Genetically modified mouse
Male
Transgene
Cellular differentiation
Blotting
Western
Bcl-X Protein
bcl-X Protein
Apoptosis
Mice
Transgenic
Bcl-2-Associated X Protein
Biology
ddc:616.07
General Biochemistry
Genetics and Molecular Biology
Mice
Bcl-2-associated X protein
Proto-Oncogene Proteins
Testis
medicine
Animals
Humans
Testosterone
Sexual Maturation
Spermatogenesis
Molecular Biology
Proto-Oncogene Proteins c-bcl-2/genetics/metabolism
Testis/cytology
Cells
Cultured
bcl-2-Associated X Protein
General Immunology and Microbiology
General Neuroscience
Apoptosis/drug effects
Cell Differentiation
Proto-Oncogene Proteins/genetics/metabolism
Sertoli cell
Testosterone/pharmacology
Spermatozoa
Spermatogonia
Cell biology
medicine.anatomical_structure
Proto-Oncogene Proteins c-bcl-2
Immunology
biology.protein
Spermatozoa/ cytology
Spermatogonia/cytology
Intracellular
Spermatogenesis/drug effects
Research Article
Zdroj: EMBO Journal, Vol. 16, No 9 (1997) pp. 2262-2270
ISSN: 0261-4189
Popis: Transgenic mice expressing high levels of the BclxL or Bcl2 proteins in the male germinal cells show a highly abnormal adult spermatogenesis accompanied by sterility. This appears to result from the prevention of an early and massive wave of apoptosis in the testis, which occurs among germinal cells during the first round of spermatogenesis. In contrast, sporadic apoptosis among spermatogonia, which occurs in normal adult testis, is not prevented in adult transgenic mice. The physiological early apoptotic wave in the testis is coincident, in timing and localization, with a temporary high expression of the apoptosis-promoting protein Bax, which disappears at sexual maturity. The critical role played by the intracellular balance, probably hormonally controlled, of the BclxL and Bax proteins (Bcl2 is apparently not expressed in normal mouse testis) in this early apoptotic wave is shown by the occurrence of a comparable testicular syndrome in mice defective in the bax gene. The apoptotic wave appears necessary for normal mature spermatogenesis to develop, probably because it maintains a critical cell number ratio between some germinal cell stages and Sertoli cells, whose normal functions and differentiation involve an elaborate network of communication.
Databáze: OpenAIRE
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