Comparison of tear proteomic and neuromediator profiles changes between small incision lenticule extraction (SMILE) and femtosecond laser-assisted in-situ keratomileusis (LASIK)
| Autor: | Louis Tong, Siew-Kwan Koh, Gary Hin-Fai Yam, Lei Zhou, Lu Deng, Molly Tzu-Yu Lin, Yu-Chi Liu, Ericia Teo, Jodhbir S. Mehta |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Proteomics Leukocyte migration genetic structures Corneal Surgery Laser medicine.medical_treatment Keratomileusis Laser In Situ Surgical Wound Keratomileusis Cornea 0302 clinical medicine Endopeptidase activity Basic and Biological Science Neuromediators Refractive surgery Myopia Postoperative Period Extracellular structure organization lcsh:R5-920 Multidisciplinary Laser-assisted in-situ keratomileusis Small incision lenticule extraction Laser assisted Receptors Neurotransmitter 030220 oncology & carcinogenesis Dry Eye Syndromes Female Lasers Excimer lcsh:Medicine (General) Adult medicine.medical_specialty Dry eye Young Adult 03 medical and health sciences Ophthalmology Endopeptidases medicine Humans Nerve Growth Factors lcsh:Science (General) ComputingMethodologies_COMPUTERGRAPHICS Wound Healing business.industry Mucins LASIK eye diseases 030104 developmental biology Tear Tears sense organs business lcsh:Q1-390 |
| Zdroj: | Journal of Advanced Research Journal of Advanced Research, Vol 29, Iss, Pp 67-81 (2021) |
| ISSN: | 2090-1224 2090-1232 |
| Popis: | Graphical abstract Introduction The tear proteomics and neuromediators are associated with clinical dry eye parameters following refractive surgery. Purpose To investigate and compare the tear proteomic and neuromediator profiles following small incision lenticule extraction (SMILE) versus laser-assisted in-situ keratomileusis (LASIK). Methods In this randomized controlled trial with paired-eye design, 70 patients were randomized to receive SMILE in one eye and LASIK in the other eye. Tear samples were collected preoperatively, and 1 week, 1, 3, 6 and 12 months postoperatively, and were examined for protein concentration changes using sequential window acquisition of all theoretical fragment ion mass spectrometry (SWATH-MS). The data were analyzed with DAVID Bioinformatics Resources for enriched gene ontology terms and over-represented pathways. Tear neuromediators levels were correlated with clinical parameters. Results Post-SMILE eyes had significantly better Oxford staining scores and tear break-up time (TBUT) than post-LASIK eyes at 1 and 3 months, respectively. Tear substance P and nerve growth factor levels were significantly higher in the LASIK group for 3 months and 1 year, respectively. SMILE and LASIK shared some similar biological responses postoperatively, but there was significant up-regulation in leukocyte migration and wound healing at 1 week, humoral immune response and apoptosis at 1 month, negative regulation of endopeptidase activity at 3 to 6 months, and extracellular structure organization at 1 year in the post-LASIK eyes. Tear mucin-like protein 1 and substance P levels were significantly correlated with TBUT (r = -0.47, r = -0.49, respectively). Conclusion Significant differences in the tear neuromediators and proteomics were observed between SMILE and LASIK, even though clinical dry eye signs have subsided and became comparable between 2 procedures. |
| Databáze: | OpenAIRE |
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