Assessment of clinical sepsis-associated biomarkers in a septic mouse model
| Autor: | Ren Huang, Xue-Jiao Li, Xi-Zhong Jing, Qiu-Ying Ye, Hang Li, Yunfeng Li, Ge Li, Yu Zhang, Shu-Hua Liu, Hui Wang, Jin-Ling Li, Huanhuan Jia |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Medicine (General) mouse model Serum albumin Aspartate transaminase clinical indicators Biochemistry Procalcitonin Sepsis 03 medical and health sciences Mice 0302 clinical medicine R5-920 Internal medicine medicine Animals Aspartate Aminotransferases Prothrombin time medicine.diagnostic_test biology business.industry Biochemistry (medical) Interleukin biomarkers Cell Biology General Medicine medicine.disease Pre-Clinical Research Reports cecal ligation and puncture (CLP) Mice Inbred C57BL Disease Models Animal 030104 developmental biology Endocrinology 030220 oncology & carcinogenesis diagnostic criteria biology.protein Cytokines Tumor necrosis factor alpha business Partial thromboplastin time |
| Zdroj: | Journal of International Medical Research, Vol 46 (2018) The Journal of International Medical Research |
| ISSN: | 1473-2300 0300-0605 |
| Popis: | Objective Clinical sepsis-associated biomarkers were utilized in a cecal ligation and puncture (CLP) septic mouse model to provide a reference for investigating pathophysiological mechanisms and evaluating novel therapeutic interventions for sepsis. Methods Sepsis in mice was induced by CLP, and clinical biomarkers were evaluated (survival rate, blood physiological and biochemical indices, cytokines, hepatorenal function parameters, and blood coagulation). Results The mortality rate was >70%. The body temperature, blood pressure, and heart rate decreased within 48 h. Low lactic acid was found at 8 h. The CLP mice showed typical inflammatory symptoms with decreased white blood cells and procalcitonin and increased levels of soluble triggering receptor expressed on myeloid cells-1, interleukin (IL)-6, IL-10, tumor necrosis factor-α, macrophage inflammatory protein (MIP)-1α, MIP-1β, and MIP-2. The platelet count and activated partial thromboplastin time significantly decreased, and the prothrombin time and prothrombin time–international normalized ratio markedly increased. Phenotypes of multiple organ dysfunction were found in the CLP model, including increased liver alanine aminotransferase and aspartate transaminase; significantly reduced total protein, globulin, and serum albumin; increased blood urea nitrogen and creatinine; and decreased blood glucose. Conclusion The clinical features of the CLP mouse model were similar to those of human patients with sepsis. |
| Databáze: | OpenAIRE |
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