Measurement of red blood cell-vitamin B12: A study of the correlation between intracellular B12 content and concentrations of plasma holotranscobalamin II
| Autor: | Glenn Tisman, J. Amin, Malcolm Brenner, S. Malkin, Mercedita Ramos, T. Browder, T. Vu, V. Cordts, G. Luszko, Raynard L. Bateman, V. Flener |
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| Rok vydání: | 1993 |
| Předmět: |
Transcobalamins
medicine.medical_specialty Erythrocytes Chemistry nutritional and metabolic diseases Hematology Cobalamin Plasma Vitamin B 12 Red blood cell chemistry.chemical_compound Endocrinology medicine.anatomical_structure Neoplasms Internal medicine Packed erythrocytes Blood plasma polycyclic compounds medicine Humans Vitamin B12 Cyanocobalamin Intracellular |
| Zdroj: | American Journal of Hematology. 43:226-229 |
| ISSN: | 1096-8652 0361-8609 |
| DOI: | 10.1002/ajh.2830430313 |
| Popis: | We have recently reported a new and rapid assay to measure plasma holotranscobalamin II (holo TC II) as a means of exploring vitamin B12 status. In order to further evaluate the significance of plasma holoTC II in determining tissue cobalamin, we have chosen the red blood cell-vitamin B12 (RBC-B12) assay as a measure of tissue vitamin B12 content and studied the relationship between RBC-B12 and plasma holoTC II levels. Plasma holoTC II and RBC-B12 concentrations were concomitantly assayed in 20 hematologically normal controls and cancer patients. In our groups of controls, the mean value of RBC-B12 was determined as 241 +/- 51 pg/ml of packed erythrocytes (PE) with a range varying from 180 to 355 pg/ml PE. Preliminary results obtained in 32 cancer patients revealed lower holoTC II and RBC-B12 levels than the control group and a required threshold value of 70 pg/ml of holoTC II in order to maintain a normal RBC-B12 greater than 180 pg/ml PE. |
| Databáze: | OpenAIRE |
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