| Popis: |
In this protocol, we describe a detailed procedure for high-resolution top-down proteomic analysis of human pancreatic and kidney tissue sections using a spatial proteomic platform by combining laser capture microdissection (LCM), microPOTS (microdroplet processing in one pot for trace samples), and nanoflow liquid chromatography mass spectrometry (LC-MS). This protocol has been applied to human pancreatic and kidney specimens for HubMAP Tissue Mapping Centers. Expected outcome: Identification of >300 proteoforms at 100-µm spatial resolution depending on sample types and LC-MS instrumentation. |
