Measurement of 5-hydroxytryptamine release in the rat medial vestibular nucleus using in vivo microdialysis
| Autor: | Yohichi Ogawa, Toshiaki Yamanaka, Shino Inoue, Taizo Kita, Hiroshi Hosoi, Toshikatsu Nakashima |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
Agonist Serotonin Microdialysis medicine.drug_class Medial vestibular nucleus Presynaptic Terminals Pharmacology Synaptic Transmission Potassium Chloride Reuptake chemistry.chemical_compound Vestibular nuclei In vivo medicine Animals Calcium Signaling Rats Wistar Neurotransmitter 8-Hydroxy-2-(di-n-propylamino)tetralin General Neuroscience Vestibular pathway Vestibular Nuclei Rats Serotonin Receptor Agonists Up-Regulation chemistry Clomipramine Extracellular Space Neuroscience Selective Serotonin Reuptake Inhibitors |
| Zdroj: | Neuroscience Letters. 323:234-238 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/s0304-3940(02)00152-0 |
| Popis: | The function of the 5-hydroxytryptamine (5-HT) system in the medial vestibular nucleus (MVN) was evaluated using KCl-evoked 5-HT release assessed by in vivo microdialysis. The release of 5-HT in the MVN was increased by the local application of KCl (100 mM) in a calcium-dependent manner. Likewise, perfusion of a 5-HT reuptake blocking agent, clomipramine (1 microM), increased extracellular levels of 5-HT in the MVN. Finally, pretreatment with the selective 5-HT(1A) agonist, 8-hydroxy-2-(di-N-propylamino) tetralin (1 mg/kg, intraperitoneally), attenuated the KCl-evoked 5-HT release. These findings suggest that 5-HT stored in vesicles is released from nerve terminals in the MVN. In addition, its regulation may be mediated by control of somatodendritic 5-HT(1A) receptor activation. |
| Databáze: | OpenAIRE |
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