The Effect of Blood Contained in the Samples on the Metabolomic Profile of Mouse Brain Tissue: A Study by NMR Spectroscopy
| Autor: | Anastasia Glinskikh, Ekaterina A. Zelentsova, Yuri P. Tsentalovich, A. E. Akulov, Olga A. Snytnikova, Maria Z. Borisova |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Magnetic Resonance Spectroscopy Metabolite Pharmaceutical Science Organic chemistry Biocompatible Materials Brain tissue 01 natural sciences Article Analytical Chemistry Specimen Handling 03 medical and health sciences chemistry.chemical_compound Mice brain tissue Metabolomics QD241-441 Drug Discovery Native state Metabolome Animals Physical and Theoretical Chemistry 030304 developmental biology Perfusion (procedure) 0303 health sciences Chloroform Chromatography Methanol 010401 analytical chemistry Brain Water Nuclear magnetic resonance spectroscopy metabolomic profile metabolomics 0104 chemical sciences nuclear magnetic resonance Blood chemistry Chemistry (miscellaneous) Molecular Medicine |
| Zdroj: | Molecules Volume 26 Issue 11 Molecules, Vol 26, Iss 3096, p 3096 (2021) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules26113096 |
| Popis: | (1) Recently, metabolic profiling of the tissue in the native state or extracts of its metabolites has become increasingly important in the field of metabolomics. An important factor, in this case, is the presence of blood in a tissue sample, which can potentially lead to a change in the concentration of tissue metabolites and, as a result, distortion of experimental data and their interpretation. (2) In this paper, the metabolomic profiling based on NMR spectroscopy was performed to determine the effect of blood contained in the studied samples of brain tissue on their metabolomic profile. We used 13 male laboratory CD-1® IGS mice for this study. The animals were divided into two groups. The first group of animals (n = 7) was subjected to the perfusion procedure, and the second group of animals (n = 6) was not perfused. The brain tissues of the animals were homogenized, and the metabolite fraction was extracted with a water/methanol/chloroform solution. Samples were studied by high-frequency 1H-NMR spectroscopy with subsequent statistical data analysis. The group comparison was performed with the use of the Student’s test. We identified 36 metabolites in the brain tissue with the use of NMR spectroscopy. (3) For the major set of studied metabolites, no significant differences were found in the brain tissue metabolite concentrations in the native state and after the blood removal procedure. (4) Thus, it was shown that the presence of blood does not have a significant effect on the metabolomic profile of the brain in animals without pathologies. |
| Databáze: | OpenAIRE |
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